结外鼻型NK/T细胞淋巴瘤裸鼠移植瘤模型LMP1蛋白检测及全长基因克隆分析

目的 研究LMP1基因和蛋白在结外鼻型NK／T细胞淋巴瘤移植瘤模型传代组织中的稳定性和变异性。方法 利用PCR和Western Blot方法检测已建立的结外鼻型NK／T细胞淋巴瘤裸鼠移植瘤模型传代组织以及供体肿瘤组织中LMP1的全长基因和蛋白表达，并将扩增测序的LMP1全长基因序列与野生型B95．8和缺失型CAO细胞株序列进行比较分析。结果 移植瘤模型传代组织均检测到LMP1基因和蛋白表达，LMP1全长序列与供体肿瘤组织同源性为99．76％。序列分析发现此LMP1基因与CAO细胞株基因序列相似，碱基序列既具相对保守性，又存在一定的特异性。结论 LMP1的基因和蛋白在结外鼻型NK／T细胞淋巴瘤裸鼠移植瘤模型传代组织中稳定存在，提示可能与该淋巴瘤的维持与发展有关。该裸鼠移植瘤模型为深入研究LMP1与此类淋巴瘤相关性提供了重要的体内实验平台。

Detecting LMP1 Protein Expression and Cloning Full-length LMP1 Gene in Extranodal Nasal Type NK/T-cell Lymphoma Transplanted Tumor Models

Objective To assess the stability and variability of the LMP1 gene and protein in nude-mice transplanted tumor models of extranodal nasal type NK/T-cell lymphoma. Methods PCR and Western Blot were performed to detect the full-length LMP1 gene and protein expression in the established extranodal nasal type NK/T-cell lymphoma transplanted tumor models and the tissues of donor tumors. Then the sequence of full-length LMP1 gene in transplanted tumor models and donor were compared with the sequence of wild-type B95. 8 and deletion-type CAO cell strains. Results LMP1 gene and protein were detected in all the extranodal nasal type NK/T-cell lymphoma transplanted tumor models, and the full-length LMP1 nucleotide sequence homology with the donor tumor was 99. 76%. It was found in sequencing analysis that LMP1 gene sequence possessed both conservative character and specificity, similar to that of CAO cell strains. Conclusion LMP1 gene and protein existed stably in the extranodal nasal type NK/T-cell lymphoma transplanted tumor models, which implied that LMP1 might relate to the maintenance and development of this lymphoma. The nude-mice transplanted tumor models provided an important experimental platform for further studies on the correlation of LMP1 and extranodal nasal type NK/T-cell lymphoma.