中俄,中哈边境地区莱姆病自然疫源地调查研究

目的：调查边境地区莱杂姆病和自然疫源地的状况。方法；对血清学、病原学、宿主、媒介，病例和地理景观方面进行调查。结果：用ＩＦＡ和ＥＬＩＳＡ法共检测人畜血清１５０１份，从１９９份人血清中检出抗体阳性１８份，阳性率为９．０５％，并发现莱姆病典型患者１５例；检查马血清２０份，阳性５份，阳性率为２５．００％；检查牛血清３９９份，阳性６９份，阳性率１７．２９％；检查羊血清８８３份，阳性１９９份，阳性率２２．５

INVESTIGATION OF LYME DISEASE FOCUS OF NATURAL INFECTION IN THE BORDER AREAS NEAR RUSSIA AND KAZAKHSTAN

Objestive: To in investigate conditions of Lyme disease and focus of natural infection in border area.Methods:Serology and etiology,host and vector, case and geographic landscape were examined.Results:1501 sera samples collected from human, horses, cattle and sheep were examined by IFA and ELISA. The positive rate of human was 9.05%(18/199), and 15 persons were found to be infected with Lyme disease. The positive rates of horses,cattle,sheep were 25.00%(5/20), 17.27%(69/399), 22.54%(199/883) respectively. 3090 ticks and 12 mice were trapped form forest,forest grassland, grassland and grassland by river. Those ticks belong to 8 species of 4 genera of 1 family. D. silvarum and D. nievus constitued the majority, the carrying rate of Borrelia burgdorferi in their mid-intestinal canal were 18.75%(6/32) and 13.33%(4/30) respectively. The ticks were divided into 86 groups. The mice were divided into 12 groups. They were cultured in BSK respectively. Borrelia burgdorferi was isolated from 2 groups of D. silvarum, 2 groups C. rufocanus and 1 group of E. talpinus. The Borrelia burgdorferi isolated was identified with multiclonal and monoclonal antibodies, the result was positive.Conclusion: the investigation of antibody of human and domestic animal sera, disease cases, Borrelia burgdorferi and its storage host, dissemination vehicle prove that the district is the focus of natural infection of Lyme disease.