| 红景天苷对活化小胶质细胞MMP-2和MMP-9表达水平的影响及其机制研究 |
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| 引用本文： | 何丽君,肖作为,鄢英慧,翁淑琴,林和辉,吴符火. 红景天苷对活化小胶质细胞MMP-2和MMP-9表达水平的影响及其机制研究[J]. 海峡药学, 2016, 0(9): 265-269. DOI: 10.3969/j.issn.1006-3765.2016.09.141 |
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| 作者姓名： | 何丽君 肖作为 鄢英慧 翁淑琴 林和辉 吴符火 |
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| 作者单位： | 1. 福建中医药大学附属人民医院药学部 福州350004;2. 湖南中医药大学长沙410208;3. 福建中医药大学福州350122 |
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| 基金项目： | 1.福建省科技厅社发处重点项目(2014Y0060)；2.全国中药特色技术传承人才培训项目(J20164811007) |
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| 摘 要： | 目的：本研究旨在探讨红景天苷对活化小胶质细胞MMP-2和MMP-9表达水平的影响作用及其可能的作用机制。方法应用LPS刺激小胶质细胞N9制备炎症细胞模型，通过MTT法筛选红景天苷的干预浓度。用红景天苷预处理细胞2h后，再加入LPS共培养小胶质细胞18h。通过ELISA法检测细胞培养上清中MMP-2和MMP-9的分泌水平，应用RT-PCR法检测活化小胶质细胞中的MMP-2和MMP-9的基因表达水平。通过EMSA法观察炎症通路上转录因子NFκB的活化水平。结果红景天苷可梯度依赖性抑制小胶质细胞活化后MMP-2和MMP-9的基因和蛋白的表达水平，同时，红景天苷可以降低NFκB的活化水平。结论红景天苷可能通过抑制明胶酶（ MMP-2、MMP-9）的表达保护血脑屏障，其机制可能与调控NF-kB通路有关。
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| 关 键 词： | 红景天苷 小胶质细胞 MMP-2 MMP-9 NFκB |
The Influence of Salidroside on the MMP-2 and MMP-9 Expression in Ac-tivated Microglia |
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| Abstract: | OBJECTIVE To investigate the influence of salidroside on the MMP-2 and MMP-9 expression in ac-tivated microglia and its underlying mechanism.METHODS MTT assay was used to confirm the effect of different concentrations of salidroside on LPS-stimulated microglial N9 cells.The protein and gene expression of MMP-2 and MMP-9 were measured using ELISA and RT-PCR analysis.The NFκB activation was analyzed using EM-SA.RESULTS The expression of MMP-2 and MMP-9 in LPS-induced microglia were significantly inhibited by sali-droside in a dose-dependent manner.In addition, Salidroside significantly reduced the activated NFκB lev-els.CONCLUSION The mechanism of neuroprotective role of salidroside maybe result from the inhibition on the MMP-2 and MMP-9 expression via suppression of the NFκB activation. |
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| Keywords: | Salidroside Microglia MMP-2 MMP-9 NFκB |
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