HPLC法测定磷酸咯萘啶注射液的含量 |
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引用本文: | 王莹,刘继华,刘屹,范亚刚. HPLC法测定磷酸咯萘啶注射液的含量[J]. 海峡药学, 2016, 0(7): 55-56. DOI: 10.3969/j.issn.1006-3765.2016.07.022 |
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作者姓名: | 王莹 刘继华 刘屹 范亚刚 |
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作者单位: | 云南省食品药品检验所昆明650011 |
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摘 要: | 目的:建立HPLC法测定磷酸咯萘啶注射液的方法。方法色谱柱为 Kromasil C18(150×4.6mm,5μm);柱温30℃;以乙腈-0.3%三乙胺溶液(磷酸调pH至3.0)(15∶85)为流动相;流速为1.0mL? min -1;检测波长为277nm。采用外标法测定磷酸咯萘啶的含量。结果磷酸咯萘啶在20.0~160.0μg? mL -1范围内,线性关系良好(r=1.0000,n=5),平均回收率为:100.3%,RSD=0.2%。结论本方法操作简单,结果准确可靠,可以有效地用于控制磷酸咯萘啶注射液的药品质量。
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关 键 词: | 磷酸咯萘啶 含量测定 HPLC |
Detemination of MaIaridine Phosphate in MaIaridine Phosphate Injection by HPLC |
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Abstract: | OBJECTIVE To establish a method for the simultaneous determination of malaridine phosphate in Malaridine Phosphate injection by HPLC .METHODS The chromatographic separation was performed on a Kroma-sil C18 column (100 ×4.6mm,5μm),the column temperature was maintained at 30℃.Acetonitrile-0.3%of three tri-ethylamine acetonitrile solution (adjusted to pH 3 with phosphoric acid)(15∶85) as the mobile phase,the flow rate of 1.0 mL? min-1 .The UV detection wavelength was 277 nm.RESULTS The linear range was within 20~160μg? mL-1 for malaridine phosphate .The recovery rate was 100.3%with RSD of 0.2%.CONCLUSION This meth-od is simple ,accurate and reliable ,which can be effectively used to control the drug quality of Malaridine Phosphate injection. |
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Keywords: | Malaridine phosphate Content determination HPLC |
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