| 丹参配方颗粒HPLC指纹图谱研究 |
| |
| 引用本文： | 郑江萍,叶政德,杨光义. 丹参配方颗粒HPLC指纹图谱研究[J]. 海峡药学, 2016, 0(9): 45-49. DOI: 10.3969/j.issn.1006-3765.2016.09.017 |
| |
| 作者姓名： | 郑江萍 叶政德 杨光义 |
| |
| 作者单位： | 湖北医药学院附属太和医院药学部十堰442000 |
| |
| 基金项目： | 2014年湖北省中青年创新团队项目(编号T201414，武当山区道地中药材GAP关键技术研究)。 |
| |
| 摘 要： | 目的：建立丹参配方颗粒的HPLC指纹图谱。方法以丹酚酸B为参照物，利用高效液相色谱梯度洗脱，测定了11批丹参配方颗粒样品；色谱柱为Waters SunFire C18（4.6mm ×250mm，5μm）；流动相：甲醇（A）-0.4%的甲酸溶液（B），检测波长270nm，柱温30℃，流速1.0mL· min -1。结果通过HPLC指纹图谱分析，标示出丹参配方颗粒16个共有色谱峰，相似度均大于0.96，并确认2个已知峰为丹酚酸 B和丹参酮ⅡA。结论建立的丹参配方颗粒的HPLC指纹图谱稳定可靠，操作简便，可为丹参配方颗粒质量控制提供科学依据。
|
| 关 键 词： | 丹参 配方颗粒 HPLC 指纹图谱 丹酚酸B 丹参酮ⅡA |
Study on the HPLC Fingerprint Chromatogram of Salvia Dispensing Gran-ule |
| |
| Abstract: | ABSTRACT:OBJECTIVE To establish an HPLC method for determination of the fingerprint of Salvia Dispensing Granule.METHODS 11 samples were analyzed by HPLC,with Salvianolic acid B as the references.Analysis was performed on a Waters SunFire C18 column ( 4.6mm ×250mm,5μm) with mobile phase A:Methanol and mobile phase B:0.4%Formic acid solution by gradient elution;flow rate was 1.0mL· min-1 ,λ=270nm,Column box tem-perature was 30℃.RESULTS By analyzing the Chromatogram fingerprint,16 peaks existed which included Salvi-anolic acid B and Tanshinone ⅡA.The similarity of 11 samples was more than 0.96.CONCLUSION The estab-lished HPLC fingerprint Chromatogram of Salvia Dispensing Granule methed is dependable and simple.This method can provide scientific basis for the quality control of Salvia dispensing Granule. |
| |
| Keywords: | Salvia Dispensing Granule HPLC Chromatogram fingerprint Salvianolic acid B TanshinoneⅡA |
| 本文献已被 万方数据 等数据库收录！ |
