| HPLC法同时测定土荆芥中槲皮素、山奈素、异鼠李素的含量 |
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| 引用本文： | 谢秋情,衷林清,王利娜,潘馨,王二丽.HPLC法同时测定土荆芥中槲皮素、山奈素、异鼠李素的含量[J].海峡药学,2016(7):65-67. |
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| 作者姓名： | 谢秋情 衷林清 王利娜 潘馨 王二丽 |
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| 作者单位： | 1. 福建中医药大学福州350122;2. 漳州卫生职业学院漳州363000 |
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| 基金项目： | 2013年度福建省中医药科研项目( wzzy201312)；福建省大学生创新项目(201410393066) |
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| 摘 要： | 目的：建立土荆芥中槲皮素、山奈素、异鼠李素含量的HPLC测定方法。方法采用 DIKMA Platisil ODS C18色谱柱（250mm ×4．6mm，5μm），流动相为乙腈－0．3％磷酸（38∶62），流速为1．0mL? min －1，检测波长为370nm，柱温为30℃。结果槲皮素进样量在0．0559～3．3552μg与峰面积线性关系良好，r＝0．9999，平均回收率为104．82％，RSD为1．83％（n ＝6）；山奈素进样量在0．1112～6．6720μg与峰面积线性关系良好，r＝0．9999，平均回收率为100．82％，RSD为2．43％（n＝6）；异鼠李素进样量在0．0128～0．7680μg与峰面积线性关系良好，r＝0．9999，平均回收率为99．49％，RSD为2．55％（n＝6）。结论本法准确、重复性好，可用于同时测定土荆芥中槲皮素、山奈素、异鼠李素的含量。
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| 关 键 词： | 土荆芥 槲皮素 山奈素 异鼠李素 高效液相色谱法 含量测定 |
SimuItaneous Determination of Quercetin,Kaempferide and Isorhamnetin in Chenopodii ambrosioidis by HPLC |
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| Abstract: | OBJECTIVE To develop an HPLC method for determination of quercetin ,kaempferide and isorham-netin in Chenopodii ambrosioidis.METHODS The DIKMA Platisil ODS C18 (250mm ×4.6mm,5μm) column was used.The mobile phase was acetonitrile-0.3%phosphoric acid(38∶62) at a flow rate of 1.0mL? min-1 and at 30℃of column temperature.The detection wavelength was 370nm.RESULTS The linear range for quercetin was 0.0559~3.3552μg,r=0.9999 (n=6).The average recovery was 104.82% and RSD was 1.83%.The linear range for kaempferide was 0.1112 ~6.6720μg, r =0.9999 ( n =6 ) .The average recovery was 100.82% and RSD was 2.43%.The linear range for isorhamnetin was 0.0128~0.7680μg,r=0.9999 (n=6).The average recovery was 99.49%and RSD was 2.55%.CONCLUSION This method is rapid and accurate ,which can be used to deter-mine the contents of quercetin ,kaempferide aod isorhamnetin in Chenopodii ambrosioidis . |
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| Keywords: | Chenopodii ambrosioidis Quercetin Kaempferide Isorhamnetin HPLC Quantitative determining |
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