HPLC法同时测定桑菊止咳合剂中绿原酸、连翘苷、黄芩苷 |
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引用本文: | 刘帅英,王慧玉. HPLC法同时测定桑菊止咳合剂中绿原酸、连翘苷、黄芩苷[J]. 海峡药学, 2016, 0(7): 49-51. DOI: 10.3969/j.issn.1006-3765.2016.07.019 |
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作者姓名: | 刘帅英 王慧玉 |
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作者单位: | 1. 浙江丽水市食品药品检验所丽水323000;2. 丽水市中医院丽水323000 |
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摘 要: | 目的:建立同时测定桑菊止咳合剂中绿原酸、连翘苷和黄芩苷的 HPLC 方法。方法色谱柱为 Agilent ZORBAX SB-C18柱(250mm ×4.6mm,5μm);流动相为甲醇-0.2%磷酸溶液,线性梯度洗脱,体积流量为1.0mL? min -1,检测波长为324、280nm,柱温35℃。结果绿原酸、连翘苷、黄芩苷的线性范围分别为0.0021~0.0471μg、0.0064~0.1269μg、0.03852~0.7703μg,平均加样回收率分别为97.8%、96.4%、99.3%。结论该方法简便、快速、准确,可作为该制剂的质量控制方法。
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关 键 词: | 桑菊止咳合剂 双波长高效液相色谱法 绿原酸 连翘苷 黄芩苷 |
SimuItaneous Determination of ChIorogenic Acid,Forsythin, and BaicaIin in Sangjuzhike Mixture by DuaI-waveIength HPLC |
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Abstract: | OBJECTIVE To establish an HPLC method for simultaneously determining chlorogenic acid , for-sythin,and baicalin in Sangjuzhike mixture .METHODS HPLC assay was carried out using an Agilent ZORBAX SB-C18 column with columntemperature at 35℃;The mobile phase was methanol-0.2% phosphoric acid solution as gradient elution with flowing rate of 1.0 mL? min-1;And the detection wavelengths were 324 and 280 nm.RESULTS The linear ranges of chlorogenic acid ,forsythin,and baicalin were 0.0021~0.0471μg,0.0064~0.1269μg and 0.03852~0.7703μg respectively ,and the average recoveries of three components were 97.8%,96.4%and 99.3%respectively.CONCLUSION The method is simple ,quick and accurate ,and may be used for the quality control of the mixture. |
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Keywords: | Sangjuzhike mixture Dual-wavelength HPLC Chlorogenic acid Forsythin Aicalin |
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