双波长HPLC法同时测定感冒灵胶囊中对乙酰氨基酚、马来酸氯苯那敏、咖啡因3种化学药物和野菊花指标性成分蒙花苷的含量

目的：建立双波长HPLC法同时测定感冒灵胶囊中对乙酰氨基酚、马来酸氯苯那敏、咖啡因3种化学药物和野菊花指标性成分蒙花苷含量的方法。方法采用岛津 Inertsil ODS－SP 柱（5μm，250mm ×4．6mm），流动相为甲醇－水－冰醋酸（26∶23∶1），流速1．0mL? min －1，检测波长260nm（对乙酰氨基酚、咖啡因和马来酸氯苯那敏）和334nm（蒙花苷），柱温35℃，进样量20μL。结果对乙酰氨基酚在109．22μg? mL －1～1092．20μg? mL －1范围内呈良好的线性关系（r＝0．9999），平均回收率为99．09％，RSD为0．95％；咖啡因在2．05μg? mL －1～20．50μg? mL －1范围内呈良好的线性关系（r＝0．9999），平均回收率为100．17％，RSD为0．85％；马来酸氯苯那敏在2．06μg? mL －1～20．59μg? mL －1范围内呈良好的线性关系（r＝1．0000），平均回收率为99．74％，RSD为1．05％；蒙花苷在9．77μg? mL －1～97．69μg? mL －1范围内呈良好的线性关系（r＝0．9998），平均回收率为98．22％，RSD为1．48％。结论该方法精密度、准确度、重复性和耐用性均较好，可作为感冒灵胶囊中4种成分的含量测定方法。

Simultaneous Determination of Paracetamol,Chlorphenamine Maleate Caffeine and Linarin in Ganmaoling Capsule by Dual-wave length HPLC

OBJECTIVE To develop a dual-wavelength HPLC method for simultaneous determining three chem-ical drugs i.e.paracetamol,chlorphenamine maleate and caffeine ,and index component of wild dendranthema flower i.e.linarin;to establish the mensuration for determining the dissolution of Ganmaoling capsule .METHODS Chro-matographic column:Shibadzu Inertsil ODS-SP column (5μm,250mm ×4.6mm),mobile phase:methanol-water-gla-cial acetic acid ( 26∶23∶1 ) , flow rate:1.0 mL? min -1 , detection wavelength:260 nm ( paracetamol , caffeine and chlorphenamine maleate ) and 334 nm ( linarin ) , column temperature:35℃, sample size:20μL.RESULTS The content of four components detected by dual-wavelength HPLC:paracetamol in 109.22~1092.20μg? mL-1 showed a good linear relationship ( r=0.9999 ) ,the average recovery rate was 99.09%,RSD was 0.95%;caffeine in 2.05~20.50μg? mL-1 showed a good linear relationship ( r=0.9999 ) , the average recovery rate was 100.17%, RSD was 0.85%;chlorphenamine maleate in 2.06~20.59μg? mL-1 showed a good linear relationship ( r=1.0000 ) , the average recovery rate was 99.74%,RSD was 1.05%;linarin in 9.77~97.69μg? mL -1 showed a good linear re-lationship ( r=0.9998 ) , the average recovery rate was 98.22%, RSD was 1.48%.CONCLUSION The estab-lished dual-wavelength HPLC method ,which can determine paracetamol ,caffeine,chlorphenamine maleate and lina-rin simultaneously , had relatively good precision , accuracy , repeatability and durability , and can be used as assay method for determining contents of the four components in Ganmaoling capsule .