离子色谱-电感耦合等离子体质谱联用法测定碘酸根和碘离子 |
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引用本文: | 陆秋艳,张文婷,张致远. 离子色谱-电感耦合等离子体质谱联用法测定碘酸根和碘离子[J]. 海峡药学, 2016, 0(9): 64-67. DOI: 10.3969/j.issn.1006-3765.2016.09.024 |
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作者姓名: | 陆秋艳 张文婷 张致远 |
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作者单位: | 1. 福建省疾病预防控制中心福州350001;2. 福建医科大学福州350122 |
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摘 要: | 目的:采用离子色谱( IC)与电感耦合等离子体质谱( ICP-MS)联用技术建立测定碘酸根和碘离子的方法。方法使用 Agilent G3154-65001分析柱和Agilent G3154-65002保护柱,通过改变色谱柱柱温,淋洗液浓度、流速,调谐 ICP-MS工作参数等,确定了碘酸根和碘离子测定的最佳条件,并在此条件下对方法的线性范围、检出限、精密度和回收率进行测试。结果40℃柱温,30mM NH4NO3淋洗液,1.2mL· min -1流速条件下,IO3-与I-检出限分别为0.10μg· L-1和0.93μg· L -1,加标回收率在93.0%~120.0%之间,相对标准偏差RSD均<5.0%。在此条件下对实际尿样进行了测定。结论实验证明该方法灵敏度和选择性高、检测结果可靠、检测快速。
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关 键 词: | 离子色谱 电感耦合等离子体质谱 碘酸根 碘离子 |
Determination of Iodate and Iodine Ion by Ion Chromatography with Induc-tively Coupled Plasma Mass Spectrometry |
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Abstract: | ABSTRACT:OBJECTIVE An analytical method was developed for separation and determination of iodate and io-dine ion using hyphenated technology of ion chromatography ( IC) and inductively coupled plasma mass spectrometry ( ICP-MS).METHODS Agilent G 3154-65001 was used as the separation column and Agilent G 3154-65002 was used as the guard column.The optimum conditions for speciation of iodate and iodine ion were summed up by chan-ging the column temperature,the concentration and flow rate of the eluent.Also ICP-MS parameters were tuned.On the basis of this method, the linear range, detection limit, precision and recovery rate of the method were test-ed.RESULTS The results showed that using 40℃as the column temperature,with 30mM NH4 NO3 and 1.2mL· min-1 as the flow rate of the eluent,the detection limits of IO3-and I -were 0.10μg· L-1 and 0.93μg· L-1 .The recoveries were between 93.0%and 120.0%.RSDs were under 5%.At last,the iodate and iodine ion were deter-mined in the urine.CONCLUSION It was showed that the method had the characteristics of high sensitivity,high selectivity,fast detection,good accuracy and precision. |
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Keywords: | IC ICP-MS Iodate Iodine |
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