| 高效液相色谱法测定复方三七胶囊中欧前胡素与异欧前胡素 |
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| 引用本文： | 朱颖,朱兴平.高效液相色谱法测定复方三七胶囊中欧前胡素与异欧前胡素[J].海峡药学,2016(9):62-64. |
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| 作者姓名： | 朱颖 朱兴平 |
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| 作者单位： | 1. 南京市食品药品监督检验院药品所南京210038;2. 四川省凉山州第一人民医院马道分院急诊科西昌615010 |
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| 摘 要： | 目的：测定复方三七胶囊中欧前胡素与异欧前胡素含量的方法。方法采用反相高效液相色谱法，Agilent 5HC-C18（2）柱，乙腈-水（44∶56）为流动相，流速为1.0mL· min -1，检测波长为300nm，柱温为35℃。结果制剂中其它组分与主峰有效分离，欧前胡素与异欧前胡素分别在1.076~21.52μg · mL -1（ r =0.9999）和0.5289~10.58μg · mL -1（ r =0.9999）线性关系良好，平均加样回收率（ n =9）为99.73%和100.15%，RSD分别为0.98%和1.00%。结论方法简便、准确，适用于测定复方三七胶囊中欧前胡素与异欧前胡素含量。
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| 关 键 词： | 高效液相色谱 复方三七胶囊 欧前胡素 异欧前胡素 |
Determination of Imperatorin and Isoimperatorin in Fufang Sanqi Capsule by HPLC |
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| Abstract: | ABSTRACT:OBJECTIVE To determine imperatorin and isoimperatorint in Fufang Sanqi Capsule.METHODS The analysis was conducted with a Agilent 5HC-C18 (2) column at 35℃by acetonitrile-water (44∶56).The detection wavelength was 300nm and the flow rate was 1mL· min-1.RESULTS Imperatorin and isoimperatorint were thor-oughly separated with other related substances.The linear ranges of imperatorin and isoimperatorint were 1.076 ~21.52μg· mL-1(r=0.9999) and 0.5289~10.58μg· mL-1 (r=0.9999),the average recoveries (n=9) were 99.73%and 100.15%with RSD of 0.98%and 1.00%.CONCLUSION This method is simple,sensitive,accu-rate,and suitable for determining imperatorin and isoimperatorint in Fufang Sanqi Capsule. |
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| Keywords: | HPLC Fufang Sanqi Capsule Imperatorin Isoimperatorin |
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