野牡丹和羊开口水提液中化学成分的HPLC-DAD-ESI-MS／MS分析

目的：采用高效液相色谱-电喷雾串联质谱法对野牡丹和羊开口水提液中化学成分进行分析比较。方法用反相 C18色谱柱进行成分分离，以甲醇（含10mM甲酸铵）-水（含10mM甲酸铵）梯度洗脱，二级管阵列检测器和电喷雾电离串联质谱在线同时检测。结果野牡丹和羊开口水提液的化学成分基本一致，均推定鉴定出含有没食子酸和（表）儿茶素，并且均主要检测出没食子酸衍生物和（表）儿茶素衍生物。结论两者水提液的主要成分均为没食子酸及其衍生物、（表）儿茶素及其衍生物，而野牡丹和羊开口传统用药均采用水煎剂，这表明两者功能与主治基本一致的原因是它们的水提液化学成分基本一致。

Determination of Water Extracts of Melastoma Candidum and Melastoma Normale by HPLC-DAD-ESI-MS/MS

OBJECTIVE The chemical composition of water extract of Melastoma candidum and Melastoma nor-male were determined by liquid chromatography-electrospray ionization tandem mass spectrometry.METHODS The high performance liquid chromatography was equipped with a reversed phase C18 column.A gradient binary mobile phase system was consisted of methanol ( containing 10 mM ammonium formate) and water ( containing 10 mM am-monium formate).Online detection were carried out by DAD and ESI-MS/MS at the same time.RESULTS The chemical composition of water extract of Melastoma candidum and Melastoma normale were basically the same.Gallic acid and ( epi) catechin were tentatively identified in water extracts of both of them,and gallic acid derivatives and (epi) catechin derivatives were the main compounds determined in water extracts of both of them.CONCLUSION Gallic acid and its derivatives,(epi) catechin and its derivatives were the main water-soluble components of bothplants.Moreover,in traditional practice both plants were used in water decoction.It indicated that the reason that thefunction and indications of both plants are basically the same derives from the fact that their water-soluble componentswere basically the same.