| 新生大鼠视神经少突胶质细胞体外培养纯化及鉴定 |
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| 引用本文： | 彭锡嘉,刘少章,叶剑,袁容娣. 新生大鼠视神经少突胶质细胞体外培养纯化及鉴定[J]. 眼科新进展, 2003, 23(3): 151-152 |
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| 作者姓名： | 彭锡嘉 刘少章 叶剑 袁容娣 |
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| 作者单位： | 1. 730050,甘肃兰州,武警甘肃总队医院眼科
2. 400042,重庆市,中国人民解放军第三军医大学大坪医院野战外科研究所眼科 |
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| 基金项目： | 国家自然科学基金资助(编号 :3 0 2 70 45 8)，重庆市科委应用基础研究资助(编号:41A115 2 C)，全军十五医药卫生科研基金资助(编号:0 1MA175)~~ |
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| 摘 要： | 目的 探讨新生2d Wistar大鼠视神经少突胶质细胞的分离方法和生长条件，为视神经损伤修复及少突胶质细胞疾病的研究奠定基础。方法 采用视神经，DMEM／F12条件培养基培养，观察细胞形态及生长，并进行免疫组织化学鉴定。结果 3d后观察．细胞自视神经周围迁出，呈圆形或梭形；11d左右，细胞基本铺满盖玻片。免疫组织化学半轧糖脑苷脂(galactocerebroside，GC)染色阳性，纯度较好。结论 本课题建立的少突胶质细胞培养方法，可获得纯化的少突胶质细胞，能满足进一步的实验需要。
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| 关 键 词： | 少突胶质细胞 视神经 细胞培养 |
| 文章编号： | 1003-5141(2003)03-0151-02 |
| 修稿时间： | 2003-04-11 |
Culture, purification and identification of oligodendrocytes of newborn rats optic nerve in vitro |
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| PENG Xi Jia,LIU Shao Zhang,YE Jian,YUAN Rong DiFrom the. Culture, purification and identification of oligodendrocytes of newborn rats optic nerve in vitro[J]. Recent Advances in Ophthalmology, 2003, 23(3): 151-152 |
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| Authors: | PENG Xi Jia LIU Shao Zhang YE Jian YUAN Rong DiFrom the |
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| Affiliation: | PENG Xi Jia,LIU Shao Zhang,YE Jian,YUAN Rong DiFrom the Department of Ophthalmology,Gansu Provincial Corps Hospital,Chinese People's Armied Police Forces,Lanzhou 730050,Gansu Province,China,Department of Ophthalmology,Daping Hospital and Research Institute of Surgery,the Third Military Medical University Chongqing 400042,China |
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| Abstract: | Objective To investigate the separation method and growth condition of oligodendrocytes of newborn Wistar rats optic nerve for further research of repair of optic nerve injury and related diseases of oligodendrocytes.Methods Optic nerves of newborn rats were used as material and DMEM/F12 chemically defined culture medium to cultivate oligodendrocytes. The morphologic and growth changes of the cells was observed, then the cells was marked by specific galactocerebroside(GC) antibody through immunohistochemical examination. Results A few of round or fusiform cells migrated from optic nerve after 3 days. The cover slips were overgrowed about 11 days later. Immunohistochemical examination showed that the cells obtained by this method can be positive cells.Conclusion Oligodendrocytes cultured by the method can be purified and meet the demands of further research. |
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| Keywords: | oligodendrocytes optic nerve cell culture |
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