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cAMP-dependent protein kinase regulates in ovo camp level of the Xenopus oocyte: Evidence for an intracellular feedback mechanism |
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| Authors: | Odile Mulner Robert Bellé René Ozon |
| Affiliation: | Laboratoire de Physiologie de la Reproduction, Groupe Stéroades ERA CNRS 694, Université Pierre et Marie Curie, 4 Place Jussieu, 75230 Paris Cedex 05 France |
| Abstract: | Microinjection of cAMP-dependent protein kinase inhibitor (1.8 μM) increases the cAMP level of Xenopus oocyte. Its effect was observed in full-grown (stage VI) as well as in vitellogenic (stage IV) oocytes. In contrast the inhibitor I1 of protein phosphatase-1 blocks cAMP accumulation. Progesterone (1 μM) decreases the cAMP level in control and in PKI-treated oocytes of both stages. These results show that cAMP concentration is regulated by a cAMP-dependent phosphorylation indicating the presence of a feedback mechanism. The feedback control is disrupted when oocyte is induced to mature by progesterone. |
| Keywords: | cAMP level cAMP-dependent protein kinase protein phosphatase-1 PKI cAMP-dependent protein kinase inhibitor phosphoprotein phosphatase-1 inhibitor-1 cAMP adenosine 3′, 5′ cyclic monophosphate IBMX 3-isobutyl-l-methylxanthine MES MPF maturation promoting factor |
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