人参瓜蒌莪术汤对小鼠Lewis肺癌端粒酶活性的影响

目的:研究人参瓜蒌莪术汤(RGE)对Lewis肺癌模型小鼠的治疗作用及机制.方法:建立小鼠Lewis肺癌移植瘤模型,分为模型对照组(NS组),环磷酰胺对照组(CP组),RGE高、中、低剂量组.在计算抑瘤率的基础上,运用PCR-ELISA法检测瘤组织端粒酶活性.结果:RGE低、中、高剂量组均能明显降低小鼠Lewis肺癌瘤重(P<0.01),中剂量组抑瘤率达47.88%;RGE低、中、高剂量组均能明显降低瘤组织细胞端粒酶的活性(P<0.01),中剂量组对端粒酶活性的抑制作用显著强于CP组(P<0.01).结论:RGE具有抑制小鼠Lewis肺癌生长的作用,其作用机制可能与降低肿瘤组织端粒酶活性有关.

Effect of Renshengualouezhu Decoction on telomerase activity of Lewis lung cancer in mice

Objective: To explore the influence of treatment and mechanism of Renshengualouezhu Decoction (RGE) on Lewis lung cancer in mice. Methods: Mice Lewis lung cancer models were established, and divided into 5 groups: model control group (NS group), low-dose RGE group, middle-dose RGE group, high-dose RGE group and Cyclophosphamide group (CP group). Then calculated the inhibition rate and used PCR-ELISA to detect the activity of telomerase. Results: The tu-mor weight of low-dose RGE group, middle-dose RGE group, high-dose RGE group and CP group were significantly de-creased (P<0.01). Inhibition rate in the middle-dose RGE group reached 47.88%. RGE could reduce the telomerase activity on all dose levels of low, middle or high (P<0.01). The middle-dose RGE group was significantly better than the CP group (P<0.01). Conclusion: RGE can significantly inhibit the growth of mice Lewis lung cancer. It may be related to its ability to reduce telomerase activity.