Biomechanical and biocompatibility characteristics of electrospun polymeric tracheal scaffolds |
| |
Authors: | Fatemeh Ajalloueian,Mei Ling Lim,Greg Lemon,Johannes C. Haag,Ylva Gustafsson,Sebastian Sjö qvist,Antonio Beltrá n-Rodrí guez,Costantino Del Gaudio,Silvia Baiguera,Alessandra Bianco,Philipp Jungebluth,Paolo Macchiarini |
| |
Affiliation: | 1. Advanced Center for Translational Regenerative Medicine (ACTREM), Department of Clinical Science, Intervention and Technology (CLINTEC), Karolinska Institutet, Stockholm, Sweden;2. University of Rome “Tor Vergata”, Department of Industrial Engineering, Intrauniversitary Consortium for Material Science and Technology (INSTM), Research Unit Tor Vergata, Rome, Italy |
| |
Abstract: | The development of tracheal scaffolds fabricated based on electrospinning technique by applying different ratios of polyethylene terephthalate (PET) and polyurethane (PU) is introduced here. Prior to clinical implantation, evaluations of biomechanical and morphological properties, as well as biocompatibility and cell adhesion verifications are required and extensively performed on each scaffold type. However, the need for bioreactors and large cell numbers may delay the verification process during the early assessment phase. Hence, we investigated the feasibility of performing biocompatibility verification using static instead of dynamic culture. We performed bioreactor seeding on 3-dimensional (3-D) tracheal scaffolds (PET/PU and PET) and correlated the quantitative and qualitative results with 2-dimensional (2-D) sheets seeded under static conditions. We found that an 8-fold reduction for 2-D static seeding density can essentially provide validation on the qualitative and quantitative evaluations for 3-D scaffolds. In vitro studies revealed that there was notably better cell attachment on PET sheets/scaffolds than with the polyblend. However, the in vivo outcomes of cell seeded PET/PU and PET scaffolds in an orthotopic transplantation model in rodents were similar. They showed that both the scaffold types satisfied biocompatibility requirements and integrated well with the adjacent tissue without any observation of necrosis within 30 days of implantation. |
| |
Keywords: | Electrospinning Tracheal scaffold Biocompatibility Cell seeding Static and dynamic cultures Correlation |
本文献已被 ScienceDirect 等数据库收录! |
|