| 头孢雷特含量测定和有关物质检查的HPLC法 |
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| 引用本文： | 宋艳霞,李波,任凤英.头孢雷特含量测定和有关物质检查的HPLC法[J].中国抗生素杂志,2010,35(1). |
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| 作者姓名： | 宋艳霞 李波 任凤英 |
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| 作者单位： | 中国医药集团四川抗菌素工业研究所有限公司,成都,610052 |
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| 摘 要： | 目的 建立头孢雷特HPLC含量测定和有关物质检查方法.方法 使用Welchrom-C_(18)柱(250mm×4.6mm,5μm),流动相为0.02mol/L磷酸二氢钠缓冲液(用磷酸调节pH至2.50)-乙腈(88:12),检测波长254nm.结果 头孢雷特在0.0102～715.4μg/ml浓度范围内有良好的线件关系(n=9),y=20230x+51110,相关系数r=0.9999,定量限为6.7ng,最低检出限为2.0ng.结论 该方法为头孢雷特含量测定和有关物质检查提供了方便、灵敏、精确、可靠的方法.
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| 关 键 词： | 高效液相色谱法 头孢雷特 含量 有关物质 |
Determination of ceforanide and its related substances by HPLC |
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| Song Yan-xia,Li Bo,Ren Feng-ying.Determination of ceforanide and its related substances by HPLC[J].Chinese Journal of Antibiotics,2010,35(1). |
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| Authors: | Song Yan-xia Li Bo Ren Feng-ying |
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| Institution: | Song Yan-xia,Li Bo , Ren Feng-ying(Sichuan Industrial Institute of Antibiotics,China Pharmaceutical Group,Chengdu 610052) |
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| Abstract: | Objective To establish an HPLC method for the determination of ceforanide and its related substances. Methods A C_(18) column (Welchrom-C_(18),250mm×4.6mm, 5μm) was used. The mobile phase, 0.02mol/L sodium dihydrogen phosphate buffered solution(adjusting with phosphate to pH2.50) : acetonitrile (88:12). The detection wavelength was 254 nm. Results The standard curve of ceforanide was linear in the range of 0.0102～715.4μg/ ml (r=0.9999). The LOQ was 6.7ng, and the LOD was 2.0ng. Conclusions The method was convenient, sensitive, accurate, and reliable for the determination of ceforanide and its related substances. |
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| Keywords: | HPLC Ceforanide Assay Related substances |
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