高效液相色谱法测定人血浆中安非他酮浓度

目的建立测定人血浆中安非他酮浓度的高效液相色谱法。方法以DiamonsilTMC18反相柱（150ramx4．6mm，5ttm）为色谱柱，流动相为30mmol·L^-1醋酸铵-甲醇（28：72V／V）；流速：1．0mL·min^-1；拄温：40℃；检测波长：250nm。提取剂为乙酸乙酯与二氯甲烷（80：20v／V）。结果安非他酮的低、中、高（2o．0，80．O，240．O斗g·L^-1）3种浓度平均相对回收率分别为102．2％，97．32％，98．17％，提取回收率分别为68．25％，71．58％，73．89％；日内、日问偏差RSD均低于15％（11=5）；分析方法的检测限5．0μg·L^-1；线性范围为10．0-320．0μg·L^-1。线性方程：Y=11．325X＋1．07，r=0．9985（n=7）。结论该方法灵敏、准确、简单、快速，可用于安非他酮临床血药浓度监测和药动学研究。

Determination of the concentration of Bupropion in human plasma by HPLC with UV detection

OBJECTIVE To establish a method for determining the concentration of bupropion in human plasma by HPLC. METHODS The drug from plasma was analyzed in a reverse phase HPLC system C1s column（ 150mm × 4. 6mm,5μm） ;mobile phase consisted of 30mmol . L^-1 ammonioum-methanol（28：72）;the flow rate was 1.0mL. min^-1; the detection wavelength was set at 250nm. Ethyl acetate and dichloromethane （80：20 V/V）was used as extracting solvent. RESULTS The average recoveries of bupropion in low, middle and high concentrations （20. 0, 80. 0,240. 0μg . L^-1 ） were 102. 2% ,97.32% and 98.17%, respectively, the extraction recovery were 68.25%, 71.58% and 73.89% ,respectively. The intra-day and inter-day variation（RSD） was less than 15% （ n = 5 ）. The calibration curve of bupropion showed good linearity,r = 0. 9985 （ n = 7 ）, over the range of 10. 0 - 320. 0μg . L^-1 . The minimum detectable concentration of bupropion was 5.01~g ~ L-1. The regression equation was Y = 11. 325X ＋ 1.07. CONCLUSION The method is sensitive,accurate, simple and quick,it can be used for clinical drug monitoring and pharmacokinetics studies of bupropion.