益肺活血颗粒对慢性肺源性心脏病家兔血管细胞因子的影响

目的观察益肺活血颗粒对慢性肺源性心脏病(CPHD)家兔相关血管细胞因子血管紧张素Ⅱ(AngⅡ)、血管内皮素1(ET-1)、肿瘤坏死因子α(TNF-α)、一氧化氮(NO)以及6酮前列腺素F1a(6-Keto-PGF1a)的影响,探讨其改善肺动脉高压的作用机制。方法采用0.7%FeCl3溶液耳缘静脉注射造模方法建立CPHD家兔模型(隔日注射1次,连续21次,共42 d,总剂量约15 mL/只),随机设立正常组、模型组、卡托普利组及益肺活血颗粒组。放射免疫法检测AngⅡ、ET-1、TNF-α和6-Keto-PGF 1a,硝酸还原酶法测定NO。结果 42 d时,模型组与正常组比较,AngⅡ、ET-1、TNF-α含量明显升高,二者有显著性差异(P<0.05);6-Keto-PGF1a、NO含量明显降低,二者有显著性差异(P<0.01)。62 d时,益气活血颗粒组与模型组比较,Ang-Ⅱ、ET-1含量明显降低(P<0.05),NO含量升高(P<0.05)。结论益气活血颗粒通过降低AngⅡ、ET-1含量,升高NO含量,舒张肺动脉血管、降低血管阻力,防治慢性肺源性心脏病。

Influences of Yifei Huoxue Granules on vascular cell factors in rabbits with chronic pulmonary heart disease

Objective To observe the influences of Yifei Huoxue Granules on relevant vascular cell factors including angiotensin Ⅱ(Ang Ⅱ),endothelin 1(ET-1),tumor necrosis factor-α(TNF-α),nitric oxide(NO) and 6-Keto-PGF1a in rabbits with chronic pulmonary heart disease(CPHD),and investigate the mechanism of the granules in relieving pulmonary hypertension.Methods The rabbit model of CPHD was established by injecting of 0.7% FeCl3 solution in ear vein(once every other day for 21 times for 42 days,and the total dose was 15 mL/each).All rabbits were randomly divided into normal group,model group,captopril group and Yifei Huoxue Granules group.The content of Ang Ⅱ,ET-1,TNF-α and 6-Keto-PGF1a were detected by using radioimmunoassay and NO content,by nitrate reductase method.Results The comparison between model group and normal group on the 42nd day showed that the content of Ang Ⅱ,ET-1 and TNF-α increased significantly(P<0.05) and content of 6-Keto-PGF1a and NO decreased significantly(P<0.01).The comparison between Yifei Huoxue Granules group and model group on the 62nd day showed that the content of Ang Ⅱ and ET-1 decreased significantly(P<0.05) and NO content increased(P<0.05).Conclusion Yifei Huoxue Granules can prevent and treat CPHD through reducing the content of Ang Ⅱ and ET-1,rising NO content,relaxing pulmonary vascular and decreasing vascular resistance.