| 银杏内酯B在血浆中的蛋白结合率研究 |
| |
| 引用本文： | 陈卫东,梁艳,谢林,王雷,吴勇,汪电雷,刘晓东.银杏内酯B在血浆中的蛋白结合率研究[J].安徽医药,2012,16(2):154-157. |
| |
| 作者姓名： | 陈卫东 梁艳 谢林 王雷 吴勇 汪电雷 刘晓东 |
| |
| 作者单位： | 安徽中医学院药学院药物代谢动力学研究室,安徽,合肥,230031;安徽省中药制剂工程技术研究中心;安徽省中药研究与开发重点实验室,安徽,合肥,230022;中国药科大学药物代谢动力学重点实验室,江苏,南京,210038;安徽省马鞍山市药品检验所,安徽,马鞍山,243000 |
| |
| 基金项目： | 2010年安徽省科技厅科技计划项目 |
| |
| 摘 要： | 目的测定银杏内酯B(ginkgolide B,GB)与大鼠和人的血浆蛋白结合率。方法将血浆样品加入内标23-羟基白桦酸,乙醚液液萃取后,高效液相色谱-质谱联用仪分析,采用平衡透析法测定GB在大鼠及人血浆中的血浆蛋白结合率。结果人和大鼠血浆中GB药物浓度在0.05～5.00 mg.L-1范围内血浆蛋白结合呈线性关系;GB与大鼠血浆蛋白结合率约为25%,与人血浆蛋白结合率约为20%。结论 GB的血浆蛋白结合率较低,在人血浆的蛋白结合率略低于大鼠,但不存在显著性差异;约80%的药物以游离态形式存在。
|
| 关 键 词： | 银杏内酯B 血浆蛋白结合率 平衡透析法 |
Protein binding ratio of Ginkgolide B in plasma |
| |
| Institution: | CHEN Wei-dong,LIANG Yan,XIE Lin,et al(1.Pharmacokinetic Laboratory in College of Pharmacy,Anhui University of Traditional Chinese Medicine,Hefei,Anhui 230031,China; 2.Anhui Province Engineering Research Center of Preparations,Hefei,Anhui 230022,China;3.Anhui Province Key Laboratory of R＆D of Chinese Medicine,Hefei,Anhui 230022,China;4.Key Lab of Drug Metabolism and Pharmacokinetics,China Pharmaceutical University,Nanjing,Jiangsu 210038) |
| |
| Abstract: | Objective To determine protein binding ratio of ginkgolide B in rat and human plasma.Methods The plasma samples which were added with internal standard（IS,23-OH Betula Alba Acid） were extracted by aether and the extraction was assayed by high performance liquid chromatography-mass spectrometry detector.The equilibrium dialysis was carried out to determine the protein binding rate of ginkgolide B in rat and human plasma.Results The binding rates of GB to rat and human plasma protein were found to be about 25% and 20%,respectively,varying with the concentration of GB from 0.05 to 5.0 mg·L-1.Conclusion The plasma protein binding rate of ginkgolide B was low and the binding rate to rat plasma was a bit higher than that to human plasma,but the difference was negligible（P0.05）.About 80% of the drugs exists in the free form. |
| |
| Keywords: | Ginkgolide B plasma protein binding ratio equilibrium dialysis |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
