| HPLC法测定肿瘤细胞中5-氟尿嘧啶的浓度 |
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| 引用本文： | 黄灿,许杜娟,夏泉,苏涌. HPLC法测定肿瘤细胞中5-氟尿嘧啶的浓度[J]. 安徽医药, 2012, 16(2): 173-174 |
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| 作者姓名： | 黄灿 许杜娟 夏泉 苏涌 |
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| 作者单位： | 安徽医科大学药学院,安徽,合肥,230032;安徽医科大学药学院,安徽,合肥,230032;安徽医科大学第一附属医院药剂科、国家中医药管理局中药化学三级实验室,安徽,合肥,230022;安徽医科大学第一附属医院药剂科、国家中医药管理局中药化学三级实验室,安徽,合肥,230022 |
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| 摘 要： | 目的建立HPLC法测定肿瘤细胞中5-氟尿嘧啶的浓度。方法采用Diamonsil C-18柱(4.6 mm×250 mm,5μm),以甲醇-水(10∶90)为流动相,流速为1.0 ml.min-1,检测波长为265 nm,柱温25℃。结果线性范围为0.056 5～1.808 mg.L-1(r2=0.9998);回收率(n=5)大于96.6%;日间、日内精密度实验的RSD(n=5)均小于8.0%。结论该试验建立的方法简便、快捷、专属性强,可用于临床研究5-氟尿嘧啶的耐药机制,也可用于5-氟尿嘧啶的药代动力学的研究。
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| 关 键 词： | 5-氟尿嘧啶 高效液相色谱 肿瘤细胞 |
HPLC determination of 5-fluorouracil in tumor cells |
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| Affiliation: | HUANG Can,XU Du-juan,XIA Quan,et al(1.School of Pharmacy,Anhui Medical University,Hefei 230032,China;2.Department of Pharmacy,Third-Grade Pharmaceutical Chemistry Laboratory of State Administration of Traditional Chinese Medicine, The First Affiliated Hospital of Anhui Medical University,Hefei 230022,China) |
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| Abstract: | Objective To establish an HPLC mothod to determine 5-fluorouracil in tumor cells.Methods The column：Diamonsil C18column（4.6 mm×250 mm,5 μm）was adopted with the mobile phase of methanol-water（10∶ 90）.The flow rate was 1.0 ml·min-1.The detection wavelength was at 265 nm,and the column temperature was 25℃.Results The calibration curves were linear between 0.0565 and 1.808 mg·L-1（r2=0.999 8）.The recovery（n=5）was more than 96.6%.Intra-day RSD and inter-day RSD were both less than 8.0%（n=5）.Conclusion The method is convenient and rapid with good specialization.It can be used for drug resistance mechanism study and pharmacokinetics study of 5-fluorouracil. |
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| Keywords: | 5-fluorouracil HPLC tumor cells |
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