黑米花青素对大鼠视网膜光化学损伤感光细胞凋亡和Caspase-1表达的影响

目的 探讨黑米花青素(black rice anthocyanins,BRACs)对视网膜光化学损伤(retinal photochemical damage,RPD)感光细胞凋亡及Caspase-1表达的影响.方法 60只Sprague-Dawley大鼠随机分为对照组(n=30)和BRACs组(n=30),均饲以基础...

The effects of black rice anthocyanins on apoptosis and caspase-1 expression in photochemical damaged retinas in rats

Objective To investigate the effects of black rice anthocyanins（BRACs） on apoptosis and expression of caspase-1 mRNA and protein in photochemical damaged retinas.Methods Sixty Sprague-Dawley rats were randomly divided into control group（n=30） and BRACs group（n=30）,and fed with basic diet with a 12 hous light-dark cycle.The BRACs group was administered with BRACs by stomach intubation at a dosage of 100 mg/kg·bw for 15 days.Meanwhile,the control group was administered with equal volume physiological saline without BRACs.Then the two groups were exposed to（3000±200） lux fluorescent light for 0-24 hous.Thereafter,the apoptosis of photoreceptors was assessed by terminal-deoxynucleoitidyl transferase mediated nick end labeling（TUNEL）,and the expression of caspase-1 mRNA and protein in photochemical damaged retinas was determined by RT-PCR and immunohistochemistry,respectively.Results The apoptotic photoreceptors were increased markedly after light exposure（P0.05）,and BRACs suppressed photoreceptors degeneration（P0.05）.The expression level of caspase-1 mRNA and protein was increased in light damaged retinas,and BRACs down-regulated caspase-1 expression（P0.05）.After light exposure,there were considerable caspase-1 positive cells,but there was a decrease in BRACs group compared to the control（P0.05）.Conclusion BRACs can inhibit photoreceptor apoptosis,which maybe is related with the down-regulation of apoptotic proteins caspase-1.