| 染料木黄酮抑制血管内皮生长因子诱导的血管内皮细胞活化及蛋白激酶K活性 |
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| 引用本文： | 韩彬,程道梅,余小平,彭晓,贾皓. 染料木黄酮抑制血管内皮生长因子诱导的血管内皮细胞活化及蛋白激酶K活性[J]. 成都医学院学报, 2011, 6(3): 192-195. DOI: 10.3969/j.issn.1674-2257.2011.03.002 |
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| 作者姓名： | 韩彬 程道梅 余小平 彭晓 贾皓 |
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| 作者单位： | 1. 遵义医学院公共卫生学院,贵州遵义563003;成都医学院公共卫生系,四川 成都610083
2. 成都医学院公共卫生系,四川 成都,610083 |
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| 基金项目： | 国家自然科学基金，四川省科技厅青年基金，成都医学院重点项目 |
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| 摘 要： | 目的 探讨染料木黄酮(genistein,Gen)抗肿瘤血管生成的分子机制.方法 实验以人脐静脉内皮细胞(human umbilical vein endothelial cells,HUVECs)为研究对象,通过锥虫蓝不相容试验测定Gen对HUVECs的细胞毒性,然后利用Caspase-3活性分析试剂盒测定Caspa...
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| 关 键 词： | 染料木黄酮 血管内皮生长因子 蛋白激酶K 人脐静脉内皮细胞 |
Genistein inhibited the activation of vascular endothelial cell and activity of its PTK induced by vascular endothelial growth factor |
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| HAN bin,CHENG Dao-mei,YU Xiao-ping,PENG Xiao-li,JIA Hao. Genistein inhibited the activation of vascular endothelial cell and activity of its PTK induced by vascular endothelial growth factor[J]. Journal of Chengdu Medical College, 2011, 6(3): 192-195. DOI: 10.3969/j.issn.1674-2257.2011.03.002 |
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| Authors: | HAN bin CHENG Dao-mei YU Xiao-ping PENG Xiao-li JIA Hao |
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| Affiliation: | 1.School of Public Health,Zunyi Medical College,Zunyi 563003,China;2.Department of Public Health,Chengdu Medical College,Chengdu 610083,China) |
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| Abstract: | Objective To investigate the molecular mechanism of genistein on anti tumor angiogenesis.Methods Cultured human umbilical vein endothelial cells（HUVECs） were choose as material.Apoptosis was quantified by using Annexin V-FITC apoptosis and caspase-3 activity assay kits.The cytotoxicity and death of HUVECs were quantified by trypan blue exclusion testing.Results Stimulation of human primary HUVECs by vascular endothelial grauth factor（VEGF） increased endothelial cell protein tyrosine kinase（PTK） activity.But treatment of ECs with genistein induced VEGF-loaded endothelial apoptosis.Conclusion Genistein interrupt the VEGF-sitmulated endothelial cell activation through inhibiting the PTK activity to play anti-angiogenesis role. |
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| Keywords: | Genistein Vascular endothelial growth Factor Protein tyrosine Kinase Human umbilical vein endothelial cells |
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