| 丝裂原活化蛋白激酶在染料木黄酮抑制血管内皮细胞中的作用 |
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| 引用本文： | 余小平,程道梅,彭晓莉,贾皓,韩彬. 丝裂原活化蛋白激酶在染料木黄酮抑制血管内皮细胞中的作用[J]. 成都医学院学报, 2011, 6(3): 186-191. DOI: 10.3969/j.issn.1674-2257.2011.03.001 |
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| 作者姓名： | 余小平 程道梅 彭晓莉 贾皓 韩彬 |
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| 作者单位： | 1. 成都医学院公共卫生系,四川成都,610083
2. 成都医学院公共卫生系,四川成都610083;遵义医学院公共卫生学院,贵州遵义563003 |
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| 基金项目： | 国家自然科学基金，四川省科技厅青年基金，成都医学院重点项目 |
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| 摘 要： | 目的 探讨染料木黄酮(genistein,Gen)抑制血管内皮细胞的分子机制.方法 人脐静脉内皮细胞(human umbilical vein endothelial cells,HUVECs)经血管内皮生长因子(VEGF)预处理后,给予1、10、100 μmol/Gen,利用酶链免疫吸附测定(ELISA)法检测基质金...
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| 关 键 词： | 染料木黄酮 血管内皮生长因子 人脐静脉内皮细胞 丝裂原活化蛋白激酶 |
The role of MAPK in inhibition of vascular endothelial cell induced by genistein |
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| YU Xiao-ping,CHENG Dao-mei,PENG Xiao-li,JIA Hao,HAN Bin. The role of MAPK in inhibition of vascular endothelial cell induced by genistein[J]. Journal of Chengdu Medical College, 2011, 6(3): 186-191. DOI: 10.3969/j.issn.1674-2257.2011.03.001 |
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| Authors: | YU Xiao-ping CHENG Dao-mei PENG Xiao-li JIA Hao HAN Bin |
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| Affiliation: | 1.Department of Public Health,Chengdu Medical College,Chengdu 610083,China;2.School of Public Health,Zunyi Medical College,Zunyi 563003,China) |
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| Abstract: | Objective To investigate the molecular mechanism underlying the activation inhibition of vascular endothelial cell induced by genistein.Methods The human umbilical vein endothelial cells（HUVECs） were treated with genistein of 1,10,100 μmol/L after treated with vascular endothelial grouth factor（VEGF）,then the expression of（MMP-2/-9 and TIMP-2/-9 were analyzed by ELISA method,MAPK expression（JNK,p38,ERK1/2） was detected with Western blot,and the collagenolytic and gelatinolytic activity were measured with collagenase assay kit and type IV collagenase assay kit.Results Stimulation of HUVECs by VEGF augmented matrix metalloproteinase-2/-9（MMP-2/-9） secretions and increased MMP-2/-9 activities,up-regulated the JNK and p38 expression.Treatment of ECs with genistein decreased production and inhibited activity of matrix metalloproteinases（MMP-2/-9）,and down-regulated the JNK and p38 expression.Conclusions Genistein interrupt the VEGF-sitmulated endothelial cell activation through inactivating MAPK,and decreasing the production of MMPs. |
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| Keywords: | Genistein Vascular endothelial grouth foutor Human umbilical vein endothelial cells Mitogen activated protein kinase |
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