丝裂原活化蛋白激酶在染料木黄酮抑制血管内皮细胞中的作用

目的 探讨染料木黄酮(genistein,Gen)抑制血管内皮细胞的分子机制.方法 人脐静脉内皮细胞(human umbilical vein endothelial cells,HUVECs)经血管内皮生长因子(VEGF)预处理后,给予1、10、100 μmol/Gen,利用酶链免疫吸附测定(ELISA)法检测基质金...

The role of MAPK in inhibition of vascular endothelial cell induced by genistein

Objective To investigate the molecular mechanism underlying the activation inhibition of vascular endothelial cell induced by genistein.Methods The human umbilical vein endothelial cells（HUVECs） were treated with genistein of 1,10,100 μmol/L after treated with vascular endothelial grouth factor（VEGF）,then the expression of（MMP-2/-9 and TIMP-2/-9 were analyzed by ELISA method,MAPK expression（JNK,p38,ERK1/2） was detected with Western blot,and the collagenolytic and gelatinolytic activity were measured with collagenase assay kit and type IV collagenase assay kit.Results Stimulation of HUVECs by VEGF augmented matrix metalloproteinase-2/-9（MMP-2/-9） secretions and increased MMP-2/-9 activities,up-regulated the JNK and p38 expression.Treatment of ECs with genistein decreased production and inhibited activity of matrix metalloproteinases（MMP-2/-9）,and down-regulated the JNK and p38 expression.Conclusions Genistein interrupt the VEGF-sitmulated endothelial cell activation through inactivating MAPK,and decreasing the production of MMPs.