佛波酯对人卵巢癌细胞株SKOV3细胞体外生长的影响

目的]探讨佛波酯（TPA）对体外培养的人卵巢癌细胞株SKOV3细胞增殖、形态、细胞周期的影响.方法]试验设试剂对照组、肿瘤细胞阴性对照组及5种不同浓度的促癌剂实验组.用四甲基偶氮唑蓝（MTT）快速比色法分析TPA不同浓度及不同时间对人卵巢癌细胞株SKOV3生长的作用;光镜下观测细胞形态学的改变;透射电镜观察细胞超微结构的变化;流式细胞仪检测细胞的凋亡及细胞周期的变化率.结果]TPA具有抑制人卵巢癌细胞株SKOV3细胞增殖的作用,呈剂量和时间依赖性.细胞周期发生G1→S期阻滞.结论]TPA能抑制SKOV3细胞增殖,显著地抑制卵巢癌细胞SKOV3的体外生长.

Effects of 12-tetradecanoyl-phorbol-13-acetate on the morphology and proliferation of human ovary cancer cell line SKOV3

Objective] This study was designed to explore the effects of TPA on the proliferation, morphology, cell cycle distribution and ultrastructure of human ovary cell line SKOV3. Methods] Grouth inhibition rates of SKOV3 cells were measured with MTT method. Cell cycle distribution and apoptosis was determined by Flow-cytometry. Morphological changes and ultramicrostructure of cells were observed with inverted phase contrast microscope and transmission electron microscope. Results] TPA could inhibited cell proliferation of human ovary cancer SKOV3 cells in a does-and time-dependent manner. Conclusions] TPA can inhibite cell proliferation of human ovary cancer skov3 cells and significantly inhibit the growth of ovary cancer cells.