Silk protein hydrolysate increases glucose uptake through up-regulation of GLUT 4 and reduces the expression of leptin in 3T3-L1 fibroblast |
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Authors: | Hyun-Sun Lee Hyun Jung Lee Hyung Joo Suh |
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Affiliation: | aInstitute of Health Science, Korea University, Seoul 136-703, Korea;bDepartment of Food and Nutrition, Korea University, Seoul 136-703, Korea |
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Abstract: | The purpose of our research was to test the hypothesis that silk protein hydrolysate increases glucose uptake in cultured murine embryonic fibroblasts. Insulin sensitizing activity was observed in a cell-based glucose uptake assay using 3T3-L1 embryonic fibroblasts. The treatment of 1 mg/mL of silk peptide E5K6 plus 0.2 nM insulin was associated with a significant increase in glucose uptake (124.0% ± 2.5%) compared to treatment with 0.2 nM insulin alone. When the 3T3-L1 cells were induced to differentiate into fibroblasts, fat droplets formed inside the cells. Silk peptide E5K6 reduced the formation of fat droplets at the 1-mg/mL dosage (86.1% ± 2.5%) when compared to the control (100.0% ± 5.8%). A 1 mg/mL dose of silk peptide E5K6 significantly increased GLUT 4 expression (131.5% ± 4.0%). The treatment of 1 mg/mL of silk peptide E5K6 did not present any changes for adipogenic expressed genes, but leptin expression was significantly increased by silk peptide E5K6 supplementation (175.9% ± 11.1%). From these results, silk peptide E5K6 increased glucose uptake via up-regulation of GLUT 4 and decreased fat accumulation via the up-regulation of leptin. |
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Keywords: | Abbreviations: 2-DG, 2-deoxy- font-variant: small-caps" >d-[1,2-3H] glucose DMEM, Dulbecco's modified Eagle's medium FAS, fatty acid synthase FBS, fetal bovine serum GLUT, glucose transporter IBMX, isobutylmethylxanthine MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide PG, pioglitazone PPAR-γ, peroxisome proliferator-activated receptor-γ SREBP-1, sterol regulatory element binding protein TG, triglyceride |
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