附子理中汤对脾阳虚大鼠ANP含量、pGCmRNA表达及胃肠动力学的影响

目的： 观察附子理中汤对脾阳虚大鼠心房利尿钠肽（ANP）含量和非可溶性鸟苷酸环化酶（pGC）mRNA表达及胃肠动力学的影响。方法： 清洁级Wistar大鼠100只随机分为对照组、模型组、附子理中汤低、中、高剂量组5组,每组20只。对照组常态饲养。模型组采用“肩胛骨间棕色脂肪组织（BAT）切除术+高脂饲料喂养+隔日寒冷环境刺激”方法造模。术后第1天喂高脂饲料共3周。在模型组基础上,附子理中汤低、中、高剂量组术后第1天按10,20, 40 g·kg^-1 ig给药,对照组及模型组则给予等容量的生理盐水,每天1次,连续3周。3周后第1天测空肠肌活动,然后取结肠标本,ELISA法检测ANP含量,RT-PCR法检测pGC mRNA表达。结果： 和对照组比较模型组的ANP含量、pGC mRNA相对表达量降低（P<0.05或P<0.01）,慢波频率显著下降（P<0.01）,快波出现频率、最大振幅、每丛快波数均增高（P<0.05或P<0.01）;纵肌收缩力振幅和环肌收缩力振幅均显著增大（P<0.01）。和模型组比较,附子理中汤高剂量组的ANP含量、pGC mRNA相对表达量升高（P<0.05或P<0.01）;附子理中汤中剂量组慢波频率上升（P<0.05）,快波出现频率、最大振幅、每丛快波数均减小（P<0.05或P<0.01）;附子理中汤高剂量组慢波频率显著上升（P<0.01）,快波出现频率、最大振幅、每丛快波数均显著减小（P均<0.01）;中剂量组纵肌收缩力振幅减小（P<0.05）,高剂量组纵肌收缩力振幅和环肌收缩力振幅均显著减小（P<0.01）。结论： 附子理中汤"止泻"的一种可能机制在于一方面上调ANP的含量及pGC mRNA表达量,进而恢复正常胃肠动力学,另一方面通过对水盐重吸收的调节作用而起效。

Effects of Fuzi Lizhong Decoction on Content of ANP,Expression of pGC mRNA and Gastrointestinal Dynamics in Rats with Spleen Yang Deficiency Syndrome

Objective: To observe effects of Fuzi Lizhong decoction on content of ANP,expression of pGC mRNA and gastrointestinal dynamics in rats with spleen Yang deficiency syndrome. Method: Hundred clean grade Wistar rats were randomly divided into control group,model group, Fuzi Lizhong decoction low-dose group, middle-dose group and high-dose group 5 groups with 20 rats in each group. The control group was given ordinary diet while model group was administrated by incising brown adipose tissue(BAT), high fatty diet and cold enviroment stimulating. From the first day after incised BAT,model group was given high fatty diet for 3 weeks, and low-dose group, middle-dose group and high-dose group were ig respectively with Fuzi Lizhong decoction (10, 20, 40 g·kg^-1) based on model group, once a day for 3 weeks. On the first day after 3 weeks the activities of jejunum muscle was observed andcolon was collected to examine content of ANP by ELISA and expression of pGC mRNA by RT-PCR. Result: Compared with the control group, in the model group, content of ANP and expression of pGC mRNA reduced(P<0.05 or P<0.01),frequency of slow wave reduced significantly(P<0.01)while occurrence frequency of fast wave, maximum amplitude, spike burst per cluster increased(P<0.05 or P<0.01), both amplitude of longitudinal muscle contraction and amplitude of circular muscle contraction enhanced significantly(P<0.01). Compared with the model group, in high-dose group, content of ANP and expression of pGC mRNA increased(P<0.05 or P<0.01).in middle-dose group frequency of slow wave increased(P<0.05) while occurrence frequency of fast wave, maximum amplitude, spike burst per cluster reduced(P<0.05 or P<0.01), in high-dose group frequency of slow wave increased significantly(P<0.01)while occurrence frequency of fast wave, maximum amplitude, spike burst per cluster reduced significantly(P<0.01), in middle-dose group amplitude of longitudinal muscle contraction reduced(P<0.05)while in high-dose group both amplitude of longitudinal muscle contraction and amplitude of circular muscle contraction reduced significantly(P<0.01). Conclusion: Fuzi Lizhong decoction could increase content of ANP and expression of pGC mRNA to restore gastrointestinal dynamics,meantime, regulate the reabsorption of water and NaCl,which perhaps is one of its mechanisms of improving symptoms of diarrhea.