Lack of F1 anti-parental resistance in H-2b/d F1 hybrids devoid of β2-microglobulin |
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Authors: | Petter Hglund Mats Y Olsson-Alheim Maria Johansson Klas Krre Charles L Sentman |
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Institution: | Petter Höglund,Mats Y. Olsson-Alheim,Maria Johansson,Klas Kärre,Charles L. Sentman |
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Abstract: | F1 hybrid mice often reject parental hematopoietic grafts, a phenomenon known as hybrid resistance. Hybrid resistance is mediated by natural killer (NK) cells and although the molecular interactions responsible for this phenomenon are largely unknown, one hypothesis suggests that parental cells are rejected because they fail to express a complete set of host major histocompatibility complex (MHC) class I molecules. Inherent in this theory is that NK cells in the F1 hybrid are instructed by self MHC class I molecules to form an NK cell repertoire capable of reacting against cells lacking these self MHC class I molecules. Here, we show that C57BL/6 x DBA/2 mice (H-2b/d) devoid of β2-microglobulin (β2m) are incapable of rejecting β2m?/? parental C57BL/6 cells (H-2b) both in vivo and in vitro. From this, we conclude that the development of an NK cell repertoire, at least in F1 mice of the H-2b/d haplotype, requires expression of MHC class I molecules complexed with β2m. |
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Keywords: | Hybrid resistance Natural killer cell β 2-microglobulin-deficient |
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