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PCR-restriction endonuclease analysis of Mycobacterium avium subsp. paratuberculosis isolates from goats, sheep, and cattle in Jordan |
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| Authors: | Nabil Hailat Adnan Fayyad Mustafa Ababneh Wael Hananeh Feth Eddine Rezig Saied Jaradat |
| Affiliation: | 1. Molecular Pathology Laboratory, Department of Pathology and Animal Health, Jordan University of Science and Technology, Irbid, Jordan 2. Department of Basic Veterinary Medical Science, Jordan University of Science and Technology, Irbid, Jordan 3. Department of Molecular Biology and Genetic Engineering, Jordan University of Science and Technology, Irbid, Jordan |
| Abstract: | Paratuberculosis is an endemic disease and induces high economical losses in Jordan. There is no information available on genotypic variation of Mycobacterium avium subsp. paratuberculosis (MAP) isolated from animals in Jordan. In this study, we investigated 150 fecal samples from sheep, goats, and cattle for the presence of paratuberculosis using bacterial culture and polymerase chain reaction (PCR)-RFLP analysis of insertion sequence IS1311. Analysis of the results revealed that genotypic information from sheep, goat, and cattle could classify them into cattle or sheep strains. All culture isolates from cattle, 12.5% of the isolates from sheep, and 50% of the isolates from goats were cattle strain, while 87.5% of the isolates from sheep and 50% of the isolates from goats were sheep strain. Sequencing of the IS1311 268?bp PCR product from the three animal species confirmed the different MAP patterns. |
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