Nestin, a neuroectodermal stem cell marker, is expressed by bovine sertoli cells |
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Authors: | Parviz Tajik Abbas Barin Mansoureh Movahedin Amir Hassan Zarnani Reza Hadavi Gholamali Moghaddam Jalil Shoja Mahmood Jeddi-Tehrani Javad Ashrafi-Helan Hamed Heidari-Vala Ebrahim Torkabadi Babak Qasemi-Panahi |
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Institution: | 1. Department of Theriogenology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran 2. Department of Clinical Pathology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran 3. Department of Anatomy, Faculty of Medical Science, University of Tarbiat Modares, Tehran, Iran 4. Nanobiotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran 5. Immunology Research Center, Iran University of Medical Sciences, Tehran, Iran 6. Monoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran 7. Department of Animal Science, Faculty of Agriculture, University of Tabriz, Tabriz, Iran 8. Department of Pathobiology, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran 9. Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran
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Abstract: | Nestin, an intermediate filament protein is expressed by neuroectodermal stem cells and tumors originating from cells of neuroectodermal and mesenchymal lineages. Nestin expression is prominent in embryos and remains upregulated until 3–6?weeks after birth but is downregulated afterward. Sertoli cells are nucleated somatic cells that are spanned in the seminiferous epithelium and play a critical role in supporting and controlling germ-cell development. In this context, we employed immunocytochemical, Western blot, and Flow cytometric analyses to demonstrate nestin expression in bovine sertoli cells. Immunostaining clearly showed that setoli cells express high levels of nestin, a result which was confirmed by Western blot analysis of purified cells. Intracellular staining of sertoli cells by flow cytometry revealed that around 74% of the cells express this marker. Given the high expression of vimentin by sertoli cells, it is proposed that the expression of nestin in these cells might be required for the formation of stable vimentin/nestin intermediate filament network. In light of these findings, it seems that sertoli cells of mature bull have potentiality of proliferation. |
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