Effects of environmental and natural estrogens on vitellogenin production in hepatocytes of the brown frog (Rana temporaria) |
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Authors: | Rouhani Rankouhi T Sanderson J T van Holsteijn I van Kooten P Bosveld A T C van den Berg M |
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Affiliation: | Institute for Risk Assessment Sciences (IRAS), Utrecht University, PO Box 80176, 3508 TD Utrecht, The Netherlands. t.rouhani@iras.uu.nl |
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Abstract: | The objective of this study was to investigate the ability of the natural estrogens and synthetic estrogens as well as the estrogen mimics to induce estrogen-receptor mediated vitellogenesis in primary hepatocytes of the brown frog (Rana temporaria). Based on EC50 values the following order was determined for the potency of the estrogens: 17beta-estradiol (EC50: 19-43 nM) approximately ethynylestradiol (EC50: 13-80 nM)>estrone (EC50: 218-241 nM)>DES (EC50: 338-3537 nM). Exposure to bisphenol A and methoxychlor concentrations up to 100 microM did not have any effect on in vitro vitellogenesis. |
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