A tumor-associated antigen in the scirrhous gastric carcinoma cell line MK-01 defined by monoclonal antibody S202 |
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Authors: | Takashi Yokota MD Takashi Masuko Toshiharu Yamaguchi Kazuya Kitamura Toshio Takahashi Yoshiyuki Hashimoto |
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Institution: | (1) The First Department of Surgery, Kyoto Prefectural University of Medicine, Kamigyo-ku, 602 Kyoto, Japan;(2) The Department of Hygienic Chemistry, Pharmaceutical Institute, Tohoku University, Sendai, Japan |
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Abstract: | A monoclonal antibody (MAb) S202, with an IgG1 isotype, that reacted strongly with the scirrhous gastric carcinoma cell line MK-01 was established. MAb S202 reacted with
the colonic cancer cell line, SW116, and the pancreatic cancer cell line, PK-1, when tested by indirect immunofluorescence.
The S202 reactive antigen was expressed in the majority of acetone-fixed fresh frozen cancer tissues. Eighty to 100 per cent
of the paraffin-embedded sections of stomach, colon and pancreatic adenocarcinoma were positive for the S202 antigen, with
diffuse cytoplasmic staining, whereas esophageal and breast cancers demonstrated markedly less immunostaining. Supplemented
serum-free medium collected from 7 day old tumor cell cultures were assayed for the presence of antibody-defined antigens.
Antigens detected by MAb S202 were released by the cell lines SW1116 and PK-1. The binding of MAb S202 to the colonic adenocarcinoma
sections was reduced after treatment with sodium periodate which suggests that respective antigenic determinants are of carbohydrate
nature without sialic acid residues. |
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Keywords: | monoclonal antibody scirrhous gastric cancer |
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