| 一种新的脱细胞阴茎海绵体基质的制备方法 |
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| 引用本文： | 崔永言,朱辉,李慧,桂耀庭,蔡志明.一种新的脱细胞阴茎海绵体基质的制备方法[J].中国微创外科杂志,2008,8(12):1137-1139. |
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| 作者姓名： | 崔永言 朱辉 李慧 桂耀庭 蔡志明 |
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| 作者单位： | 1. 北京大学深圳医院整形外科,深圳,518036
2. 北京大学深圳医院男性生殖医学实验室,深圳,518036 |
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| 基金项目： | 深圳市科技局重点基金
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广东省医学科学技术研究基金
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| 摘 要： | 目的探索一种新的脱细胞阴茎海绵体基质的制备方法。方法取健康壮年兔完整阴茎海绵体组织，以Triton-X100与NH3·H2O（氨水）联合提取法进行脱细胞处理。标本作HE染色，组织学观察分析脱细胞效果。结果脱细胞处理25天后，成功获得脱细胞海绵体基质。所得基质外观良好。HE染色观察无细胞存在，弹力纤维排列规整，间隙较大，结构无破坏。结论利用Triton-X100与NH3·H2O联合提取法可成功制备完整无细胞阴茎海绵体基质。
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| 关 键 词： | 脱细胞海绵体基质 阴茎海绵体 基质 |
New Method to Prepare Acellular Matrix of the Penile Cavernous Body |
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| Cui Yongyan,Zhu Hui,Li Hui,et al..New Method to Prepare Acellular Matrix of the Penile Cavernous Body[J].Chinese Journal of Minimally Invasive Surgery,2008,8(12):1137-1139. |
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| Authors: | Cui Yongyan Zhu Hui Li Hui |
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| Institution: | Cui Yongyan,Zhu Hui,Li Hui,et al.Department of Plastic Surgery,Shenzhen Hospital of Peking University,Shenzhen 518036,China |
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| Abstract: | Objective To find a new method to prepare the acellular matrix of the penile cavernous body. Methods Intact specimens of the penile cavernous body were collected from healty adult rabbits, treated with Triton-X100 and NH3 · H2O, and then stained with HE. Light microscopy was employed to observe the acellular ceils. Results Acellular matrix of the penile cavernous body was obtained after a 25-day treatment with Triton-X100 and NH3 · H2O. The matrix was intact. HE staining showed that the matrix was composed of regularly arranged elastic fibers with wide intra-spaces without any ceils inside. Conclusion Acellular matrix of the penile cavernous body can be prepared by using Triton-X100 and NH3 · H2O. |
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| Keywords: | Acellular matrix Penile cavernous body Matrix |
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