|
|||||
|
|
| 金叶败毒制剂对人巨细胞病毒感染人胚肺成纤维细胞增殖及凋亡的影响 | |
| 引用本文： | 袁慧,刘璐,闻良珍.金叶败毒制剂对人巨细胞病毒感染人胚肺成纤维细胞增殖及凋亡的影响[J].中国妇幼保健,2010,25(3). |
| 作者姓名： | 袁慧 刘璐 闻良珍 |
| 作者单位： | 1. 江汉大学医学院,湖北,武汉,430056 2. 华中科技大学同济医学院附属同济医院药剂科 3. 华中科技大学同济医学院附属同济医院妇产科 |
| 基金项目： | 国家自然科学基金资助项目(30271673); 湖北省自然科学基金资助项目(2007ABA267); 湖北省教育厅资助项目(B200734001) |
| 摘 要： | 目的:探讨中药金叶败毒制剂(简称中药)对人巨细胞病毒(HCMV)感染人胚肺成纤维细胞(HEL)增殖及凋亡的影响。方法:用HCMVAD169感染人胚肺成纤维细胞,分别用金叶败毒制剂和更昔洛韦(GCV)干预后,采用MTT法检测HCMV感染及药物干预后HEL细胞的增殖活性,并采用流式细胞术检测HEL、HCMV感染及药物干预后细胞凋亡指数。结果:在HCMV感染48h内,细胞增殖及凋亡指数与细胞对照组无明显差异。HCMV感染72h后,细胞增殖明显受抑制,凋亡指数明显增高;而两种药物干预后,72h后明显促进细胞增殖,抑制细胞凋亡。结论:金叶败毒制剂可能通过促进HCMV感染细胞的增殖,抑制感染细胞的凋亡而发挥抗病毒作用。 |
| 关 键 词： | 金叶败毒制剂 巨细胞病毒 增殖活性 凋亡指数 |
Effect of Jinyebaidu preparation on proliferation and apoptosis of human embryonic lung fibroblast cells infected by human cytomegalovirus |
|
| YUAN Hui,LIU Lu,WEN Liang-Zhen.Effect of Jinyebaidu preparation on proliferation and apoptosis of human embryonic lung fibroblast cells infected by human cytomegalovirus[J].Maternal and Child Health Care of China,2010,25(3). | |
| Authors: | YUAN Hui LIU Lu WEN Liang-Zhen |
| Institution: | YUAN Hui,LIU Lu,WEN Liang-Zhen.Medical College,Jianghan University,Wuhan 430056,Hubei,China |
| Abstract: | Objective:To explore the effect of Jinyebaidu preparation on proliferation and apoptosis of human embryonic lung fibroblast cells infected by human cytomegalovirus (HCMV).Methods:The human embryonic lung fibroblast cells were infected by HCMVAD169,then intervened by Jinyebaidu preparation and ganciclovir; MTT method was used to detect HCMV infection and proliferative activity of human embryonic lung fibroblast cells after intervention; human embryonic lung fibroblast cells infection,HCMV infection and apopt... |
| Keywords: | Jinyebaidu preparation Cytomegalovirus Proliferative activity Apoptosis index |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |