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HPLC法测定复方红根草片中蒙花苷含量
引用本文:乔丽,王静,袁子民. HPLC法测定复方红根草片中蒙花苷含量[J]. 中国现代药物应用, 2010, 4(1): 16-17
作者姓名:乔丽  王静  袁子民
作者单位:1. 辽宁省大连远大制药有限公司,116021
2. 辽宁中医药大学药学院
摘    要:目的建立HPLC法测定复方红根草片中蒙花苷含量。方法采用Diamonsil C18色谱柱,以乙腈-水(23:77)为流动相,检测波长334nm。结果蒙花苷在0.059~0.295μg范围内线性关系良好,r=0.9994,平均回收率为98.2%(n=6),RSD为1.4%。结论本法简便、准确、重复性好,可用于复方红根草片中蒙花苷含量测定。

关 键 词:复方红根草片  蒙花苷  HPLC

Determination of linarin in Fufanghonggencao Tablet by RP-HPLC
QIAO Li,WANG Jing,YUAN Zi-min. Determination of linarin in Fufanghonggencao Tablet by RP-HPLC[J]. Chinese Journal of Modern Drug Application, 2010, 4(1): 16-17
Authors:QIAO Li  WANG Jing  YUAN Zi-min
Affiliation:.(Dalian YuanDa Pharmaceutical Co.Ltd.2.School of Pharmacy,Liaoning University of TCM,Dalian,116600,China )
Abstract:Objective To establish an HPLC method for determination of linarin in Fufanghonggencao Tablet.Methods An HPLC method was developed.The chromatographic conditions were as follow:Diamonsil C18 column and acetonitrile-water(23:77)as mobile phase,detection wavelength at 334 nm.Results The linear range of linarin was 0.059-0.295 μg.The average recovery rate was 98.2% and RSD was 1.4%.Conclusion The method is simple and accurate,with good repeatability,and can be used for determination of linarin in Fufanghonggencao Tablet.
Keywords:HPLC
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