TTV感染的肝组织病理学特点及病毒DNA原位检测

目的 观察单一ＴＴＶ（ｔｒａｎｓｆｕｓｉｏｎ ｔｒｓｎｓｍｉｔｔｅｄ ｖｉｒｕｓ）急性感染患者肝组织病理损害特点及病毒ＤＮＡ的表达。方法 对某职业学校ＴＴＶ感染流行期间，１８例血清ＴＴＶ阳性住院患者的肝活检组织进行ＨＥ、浸银Ｇｏｍｏｄｒｉ染色检查，以及地高辛标记ＴＴＶ ＤＮＡ（Ｄｉｇ－ＴＴＶ ＤＮＡ）探针原位杂交。结果 １８例肝穿刺标本中出现汇管区炎１４例（７７．８％），胆小管损害６例（３３．４％），肝小叶内灶状坏死７例（３８．９％），微脂滴３例（１６．７％），汇管区纤维化２例（１１．１％），原位杂交阳性８例（４４．４％），ＴＴＶ阳性表达细胞散在分布于肝小叶内，坏死区及汇管区旁较密集。结论 单一ＴＴＶ急性感染可致患者肝组织轻度炎性病变，其特征是汇管区炎及胆小管损害。ＴＴＶ可能是一种嗜肝病毒。

Hepatic histological characteristics of TTV hepatitis and detection of the virus by in situ hybridization

Objective To observe the histological characteristics and presence of transfusion transmitted virus (TTV) in the liver tissues. Methods Eighteen cases of paraffin embedded liver tissues of the hospitalized patients with TTV infection were examined with routine and special stains and in situ hybridization with Dig-TTV DNA probe. Results Histological alterations were characterized as portal inflammation (77.8%), interlobular bile duct lesions (33.4%), focal necrosis (38.9%), microvesicular fatty degeneration (16.7%) and fibrosis of the portal tracts (11.1%). Out of the 18 cases, TTV DNAs was detected in 8 cases (44.4%), in which the positive cells were scattered in the intralobular areas and more aggregated in the periportal and necrotic areas. Conclusion TTV, a novel hepatotropic virus, may be solely responsible for the mild hepatitis, characterized by infiltration in portal tract and duct lesions in the interlobular bile.