HGV/GBV-C与HCV混合感染者肝组织中相关病毒表达的免疫组化研究

目的了解HGV／GBV-C与HCV混合感染者肝组织中HGV/GBV-C相关抗原的分布状况，探讨HGV/GBVC对肝脏的损害机制。方法以抗HGV／GBV-CNS5单克隆抗体或抗HCVNS3单克隆抗体为试剂，采用免疫组织化学方法检测肝炎病人肝组织中HGV/GBV-C、HCV相关抗原表达。结果56例肝炎病人肝组织中HGV/GBV-C相关抗原表达阳性率为26.79％(15／56)；HCVNS3抗原表达阳性率为39.29％(22/56)。HGV/GBV-CNS5抗原表达阳性信号主要位于肝细胞胞浆中，染色阳性细胞周围可见淋巴细胞浸润。结论肝细胞中存在HGV／GBV-C相关抗原表达，其编码产物可能作为一种靶抗原，诱发免疫病理反应，免疫损伤可能是其发病机制之一。

Immunohistochemical Studies of HGV/GBV-C and HCV-related Antigens in the Liver Tissues of the Patients Co-infected with Hepatitis C and G Viruses

Objective To investigate the distribution of hepatitis G virus (HGV)/GB virus C (GBV-C) related antigens in the liver tissues of the patients co infected with hepatitis C and G viruses and to discuss underlying mechanism of liver damages induced by HGV/GBV C. Methods HGV/GBV-C or HCV related antigens were detected with immunohistochemical methods, by using NS 5 and NS 3 monoclonal antibodies, in paraffin embedded liver tissues. Results Positive rate of HGV/GBV C related antigens was 26.79% (15/56), and HCV NS3 39.29% (22/56) in 56 cases of the hepatitis. Expression of HGV/GBV C NS5 antigen mostly limited in the cytoplasm of liver cells, which was surrounded by lymphocytic infiltration. Conclusion HGV/GBV C related antigens are expressed in the liver cells. HGV/GBV C encoded product may be a target antigen , which induces immune reactions. The immune damage could be one of the pathogeneses of the hepatitis.