| Institution: | 1.Division of Thoracic Surgery, Department of Surgery,University of Pennsylvania and Philadelphia VA Medical Center,Philadelphia,USA;2.Department of Pathology, Perelman School of Medicine,University of Pennsylvania,Philadelphia,USA;3.Departments of Biomedical Engineering and Chemistry,Emory University,Atlanta,USA;4.Department of Chemistry,Purdue University,West Lafayette,USA;5.Division of Thoracic Surgery,University of Pennsylvania School of Medicine,Philadelphia,USA |
| Abstract: | PurposeDuring lung surgery, identification of surgical margins is challenging. We hypothesized that molecular imaging with a fluorescent probe to pulmonary adenocarcinomas could enhance residual tumor during resection.ProceduresMice with flank tumors received a contrast agent targeting folate receptor alpha. Optimal dose and time of injection was established. Margin detection was compared using traditional methods versus molecular imaging. A pilot study was then performed in three humans with lung adenocarcinoma.ResultsThe peak tumor-to-background ratio (TBR) of murine tumors was 3.9. Fluorescence peaked at 2 h and was not improved beyond 0.1 mg/kg. Traditional inspection identified 30 % of mice with positive margins. Molecular imaging identified an additional 50 % of residual tumor deposits (p?<?0.05). The fluorescent probe visually enhanced all human tumors with a mean TBR of 3.5.ConclusionsMolecular imaging is an important adjunct to traditional inspection to identify surgical margins after tumor resection. |
