| 变性高效液相色谱对CTX-M型超广谱β内酰胺酶基因分型研究 |
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| 引用本文： | 段辉丽,刘文恩,陈腊梅,李虹玲,潘军,邹明祥,许力.变性高效液相色谱对CTX-M型超广谱β内酰胺酶基因分型研究[J].中华检验医学杂志,2002,32(1):678-682. |
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| 作者姓名： | 段辉丽 刘文恩 陈腊梅 李虹玲 潘军 邹明祥 许力 |
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| 作者单位： | 中南大学湘雅医院检验科,长沙,410008;英国伯明翰大学医学院; |
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| 摘 要： | Objective To investigate the genotype distribution of CTX-M-type extended-spectrum β-1actamase(ESBLs) by denaturing high-performance liquid chromatography(DHPLC) in Hunan Province and the accuracy of DHPLC assay. Methods The blaCTX-M genes of standard strains and clinical ESBLs-producing Enterobacteriaceae were amplified by multiplex PCR followed by DHPLC and genotype determination. 25 isolates randomly selected were sequenced to assess the accuracy of DHPLC method. Results Among 142 ESBLs-producing isolates, 109 isolates carried blaCTX-M gene (76. 8% ). Four different CTX-M genotypes were detected by DHPLC, including CTX-M-3 (33 isolates), CTX-M-15 (19 isolates), CTX-M-14 (52 isolates) and CTX-M-9 (5 isolates). The DHPLC typing of 25 isolates suggested that 24 isolates were verified uniformly by the sequencing, but one CTX-M-15 isolate typed by DHPLC was shown to be CTX-M-82 by sequencing. Conclusion DHPLC is a powerful tool for genotyping of the resistance gene and is worth being applied in the clinical and scientific research with accurate, rapid and economic advantages.
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| 关 键 词： | β内酰胺酶类 大肠杆菌蛋白质类 色谱法 高压液相 基因型 |
Genotype of CTX-M-type extended-spectrum beta-lactamases by denaturing high-performance liquid chromatography |
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| DUAN Hui-li,LIU Wen-en,CHEN La-mei,LI Hong-ling,PAN Jun,ZOU Ming-xiang,XU Li.Genotype of CTX-M-type extended-spectrum beta-lactamases by denaturing high-performance liquid chromatography[J].Chinese Journal of Laboratory Medicine,2002,32(1):678-682. |
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| Authors: | DUAN Hui-li LIU Wen-en CHEN La-mei LI Hong-ling PAN Jun ZOU Ming-xiang XU Li |
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| Abstract: | |
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| Keywords: | beta-LactamasesEscberichia coli proterinsChromatography high pressure liquidGenotype |
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