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提纯孕马血清促性腺激素的中试生产研究
引用本文:杨兴辉,王秀军,柴希文,尤建东,崔青山,董仁培.提纯孕马血清促性腺激素的中试生产研究[J].中国生化药物杂志,1997(1).
作者姓名:杨兴辉  王秀军  柴希文  尤建东  崔青山  董仁培
作者单位:兰州军区后勤部马场管理局农牧研究所!山丹,734104(杨兴辉,王秀军,柴希文,尤建东,崔青山),兰州军区后勤部(董仁培)
摘    要:孕马血清经偏磷酸、乙醇分级沉淀得比活为409IU/mg的粗品,回收率为47.3%。粗品经离子交换层析得到比活为1455IU/mg的精品,回收率达97.4%。再过柱层析得比活为2400IU/mg的产品。

关 键 词:孕马血清促性腺激素  提纯  中试生产

The Study of Middle Scale Production of Pregnant Mare Serum Gonadotrophin
Yang Xinghui, Wang Xiujun, Chai Xiwen, You Jiandong, Cui Qingshan, Dong Renpei.The Study of Middle Scale Production of Pregnant Mare Serum Gonadotrophin[J].Chinese Journal of Biochemical Pharmaceutics,1997(1).
Authors:Yang Xinghui  Wang Xiujun  Chai Xiwen  You Jiandong  Cui Qingshan  Dong Renpei
Institution:Yang Xinghui; Wang Xiujun; Chai Xiwen; You Jiandong; Cui Qingshan; Dong Renpei(Farming-Animal Husbandy Insititute Machong Manage Bureau Lanzhou Rear-Serrice Department (Shendan 734104
Abstract:The pregnant mare serum was experienced in HpO3, alcohol precipitation. Activity of the inirial productobtained 409 IU/mg and the rate of recovery in yields was 47. 3%. The initial product was experienced by ion-exchenged chromatrograply. Activity of the find ion-exchenged chromatrograply. Activity of the find product 1 455 IU/mg. And the rate of recovery in the yields was 97.4 %. The activity of the pure product 2 400 IU/mg by columm chromatography.
Keywords:PNSG  Extraction  Middle trialproduct
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