Inhibition of ethanol-induced platelet activation by agents that elevate cAMP |
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Authors: | Raphael Rubin and Jan B. Hoek |
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Affiliation: | Department of Pathology and Cell Biology Jefferson Medical College Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA |
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Abstract: | Ethanol activates phosphoinositide-specific phospholipase C in human platelets resulting in the mobilization of intracellular calcium and shape change (Arch. Biochem. Biophys. 260, 480–492, 1988). Preincubation of platelets with agents that increase levels of cAMP (i.e., forskolin,prostaeyclin) inhibited these responses to ethanol in a concentration- and time-dependent manner. The inhibitory effect was potentiated by the phoshpodiesterase inhibitor, isomethybutylxanthine. When added after ethanol, these agents also reversed platelet shape change and lowered cytosolic free calcium to basal levels. The results demonstrate a direct inhibitory effect of cAMP on the ethanol-induced activation of phospholipase C. |
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Keywords: | Author Keywords: platelet ethanol activation shape calcium cAMP |
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