| 人幽门螺杆菌热休克蛋白A亚单位的基因克隆及序列分析 |
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| 引用本文： | 郭红,邹全明,赵晓晏,毛旭虎,吴超.人幽门螺杆菌热休克蛋白A亚单位的基因克隆及序列分析[J].免疫学杂志,2001,17(3):185-188. |
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| 作者姓名： | 郭红 邹全明 赵晓晏 毛旭虎 吴超 |
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| 作者单位： | 1. 第三军医大学新桥医院消化内科,
2. 第三军医大学临床微生物学教研室, |
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| 基金项目： | 国家“九五”重点攻关课题! (96 - 90 1- 0 1- 5 4)，全军“九五”医药卫生科研基金! (98D0 0 4)资助项目 |
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| 摘 要： | 目的 获取人幽门螺杆菌（helicobacter pylori,Hp)热休克蛋白A基因（hsqA)进行核苷酸序列分析。方法 利用PCR技术扩增HspA的DNA，并将其定向插入PinPoint^TMXa-3载体中进行核苷酸序列分析。结果 大气层克隆的HspADNA序列与GeneBank公布的序列有15个碱基存在差异。同源95.8%。结论 所克隆的HspA基因可用于重组表达及相关研究。
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| 关 键 词： | 幽门螺杆菌 热休克蛋白A 序列分析 亚单位 基因克隆 |
| 文章编号： | 1000-8861（2001）03-0185-04 |
| 修稿时间： | 2001年1月9日 |
Clone and sequence analysis of human Helicobacter pylori heat shock protein A gene |
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| GUO Hong ,ZOU Quan-ming ,ZHAO Xiao-yan ,MAO Xu-hu ,WU Chao WTBX.Clone and sequence analysis of human Helicobacter pylori heat shock protein A gene[J].Immunological Journal,2001,17(3):185-188. |
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| Authors: | GUO Hong ZOU Quan-ming ZHAO Xiao-yan MAO Xu-hu WU Chao [WTBX] |
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| Institution: | GUO Hong 1,ZOU Quan-ming 2,ZHAO Xiao-yan 1,MAO Xu-hu 2,WU Chao 2 [WT5BX] |
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| Abstract: | ObjectiveTo obtain DNA of human Helicobacter pylori (Hp) heat shock protein A(hspA), and investigate the nucleotide sequence. MethodsThe HspA DNA was amplified by PCR from the chromosomal DNA of clinical isolated Helicobacter pylori and inserted into PinPoint TM Xa-3 Then the sequence was analysed. ResultsThe HspA gene's nucleotide homology was 95 8% as compared to that in Genebank. ConclusionA confirmed HspA gene has been obtained, thus a good foundation for recombination, expression and associated research laid. |
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| Keywords: | helicobacter pylori heat shock protein A sequence analysis |
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