| 珍稀药用金钗石斛组培苗遗传稳定性直接扩增长度多态性检测 |
| |
| 引用本文： | 李标,唐坤,朱祥先.珍稀药用金钗石斛组培苗遗传稳定性直接扩增长度多态性检测[J].时珍国医国药,2010,21(4). |
| |
| 作者姓名： | 李标 唐坤 朱祥先 |
| |
| 作者单位： | 1. 重庆邮电大学,生物信息学院,重庆,400065;中国医学科学院药用植物研究所生物技术中心,北京,100193
2. 重庆邮电大学,生物信息学院,重庆,400065 |
| |
| 基金项目： | 重庆市自然科学基金，重庆市教委科技研究基金，重庆邮电大学博士启动基金资助 |
| |
| 摘 要： | 目的 研究珍稀药用金钗石斛DNA指纹图谱,初步探讨直接扩增长度多态性(DALP)分子标记技术在石斛组织培养过程中遗传稳定性检测上的应用.方法 采用直接扩增长度多态性(DALP)技术对金钗石斛野生移栽苗和组培苗进行基因组DNA多态性分析.结果 从12对引物组合中筛选出6对能获清晰条带的引物组合,分别利用琼脂糖和聚丙烯酰胺对DALP-PCR产物进行凝胶电泳,发现两组苗扩增的DNA带型基本一致,未发现明显变异,说明金钗石斛茎尖离体继代培养具有较高的遗传稳定性.结论 DALP分子标记技术可用于石斛遗传稳定性和遗传多样性研究.
|
| 关 键 词： | 金钗石斛 组培苗 直接扩增长度多态性 遗传稳定性 指纹图谱 |
DALP Analysis of Genetic Stability of Dendrobium nobile Tissue Culture Seedling |
| |
| LI Biao,TANG Kun,ZHU Xiang-xian.DALP Analysis of Genetic Stability of Dendrobium nobile Tissue Culture Seedling[J].Lishizhen Medicine and Materia Medica Research,2010,21(4). |
| |
| Authors: | LI Biao TANG Kun ZHU Xiang-xian |
| |
| Institution: | LI Biao1,2,TANG Kun1,ZHU Xiang-xian1 (1.College of Bioinformation,Chongqing University of Posts & Telecommunications,Chongqing 400065,China,2.Center of Biotechnology,Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences,Beijing 100193,China) |
| |
| Abstract: | Objective To study DNA fingerprint of Dendrobium nobile and application of direct amplification of length polymorphism(DALP) to the identification of genetic stability on the course of tissue culture.Methods The polymorphism of transplanting wild seedlings and tissue culture seedlings from D.nobile were detected by DALP technique.Results Six pairs of primer combinations screened from 12 pairs of primer combinations might amplify clear bands.By agarose and polyacrylamide gel electrophoresising,respectively,t... |
| |
| Keywords: | Dendrobium nobile Tissue culture seedling DALP Genetic stability Fingerprinting |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
