缺氧对高原藏族、汉族人脐静脉内皮细胞VEGF、iNOS和eNOS mRNA表达影响的比较

目的：比较缺氧对世居高原藏族人和移居高原汉族人脐静脉内皮细胞（UVECs）血管内皮生长因子（VEGF）、诱导型一氧化氮合酶（iNOS）和内皮型一氧化氮合酶（eNOS） mRNA表达的影响。 方法： 分离脐静脉内皮细胞，体外原代培养，分为①世居高原藏族组和②移居高原汉族组两组，每组包括常氧对照和缺氧(0.5% O2)2 h、4 h、12 h和24 h时点。分离细胞总RNA，分别用RT-PCR检测VEGF、iNOS和eNOS mRNA表达。 结果： 缺氧可以诱导世居高原藏族人UVECs表达iNOS和VEGF mRNA表达增加，同时抑制eNOS mRNA表达。移居高原汉族人UVECs表达上述基因mRNA的特点与世居高原藏族人相似。 结论： 缺氧时世居高原藏族人UVECs VEGF、iNOS和eNOS mRNA表达特点与移居高原汉族人相似，提示上述基因表达的改变可能是缺氧反应机制的共同过程。

Effects of hypoxia on expression of VEGF, iNOS and eNOS mRNA in human umbilical vein endothelial cells from Tibetan and Han

AIM: To compare the effects of hypoxia on expression of vascular endothelial growth factor (VEGF), iNOS and eNOS mRNA in cultured umbilical vein endothelial cells (UVECs) obtained from Tibetan and Han. METHODS: UVECs were obtained from native Tibetan and immigrant Han, respectively and cultured under hypoxia conditions (0.5% oxygen) for 2 h, 4 h, 12 h, and 24 h and normoxic conditions. VEGF, iNOS and eNOS mRNAs were detected with methods of RT-PCT. RESULTS: VEGF and iNOS mRNAs were up-regulated while eNOS mRNA depressed by hypoxia similarly in Tibetan and Han UVECs. CONCLUSION: Our results suggest that the changes of VEGF, iNOS and eNOS mRNA expression are common pathways in the mechanisms of hypoxic responses.