Resistance to gram-negative organisms due to high-level expression of plasmid-encoded ampC β-lactamase blaCMY-4 promoted by insertion sequence ISEcp1 |
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Authors: | Ryuichi Nakano Ryoichi Okamoto Noriyuki Nagano and Matsuhisa Inoue |
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Institution: | (1) School of Medicine and Environmental Infectious Diseases, Graduate School of Medical Sciences, Kitasato University, 1-15-1 Kitasato, Sagamihara Kanagawa, 228-8555, Japan |
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Abstract: | A Klebsiella pneumoniae strain, KU6500, which showed resistance to extended-spectrum β-lactams and produced the plasmid-encoded AmpC β-lactamase
CMY-4, was identified from clinical isolates in Japan. The aim of this study was to identify the mechanism of the high-level
expression of bla
CMY-4. Sequence analysis indicated that the promoter element of Citrobacter freundii was conserved, but the insertion sequence ISEcp1 coding with the putative promoter element, was inserted into the AmpR binding site. We determined the influence of the promoter
on bla
CMY-4 expression and β-lactam resistance. Two recombinant plasmids containing the entire bla
CMY-4 gene, with or without the ISEcp1-mediated promoter sequences, were constructed and named pMWampC and pMWISEcp1, respectively. Escherichia coli DH5α (pMWISEcp1) was resistant to almost all β-lactams tested and E. coli DH5α (pMWampC) was susceptible to all, except for cephalothin. In addition, the activity of each promoter was measured by
subcloning the element into a promoterless luciferase plasmid pGL3-Basic vector. The expression of the putative promoter of
ISEcp1 was 18.9-fold higher than that of C. freundii. These results suggest that the putative promoter element of ISEcp1 is necessary for the high-level expression of bla
CMY-4 to confer resistance to extended-spectrum cephalosporins. |
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Keywords: | ISEcp1 Plasmid-encoded AmpC β -lactamase bla CMY-4 Citrobacter freundii |
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