活性染色质诱导抗核抗体生成及肾损伤

系统性红斑狼疮 (SLE )的病因和诱导抗核抗体 (ANA )生成的激发原迄今不明。本实验试图用ConA活化淋巴细胞的染色质免疫同系BALB/c小鼠 ,寻找诱导ANA生成的真正免疫原 ,阐明SLE发生的激发原是自身活化细胞的核成分 ,而且证明它所诱导的ANA具有致病性。从ConA活化的脾细胞中提取染色质 ,然后免疫同系BALB/c小鼠 ,用ELISA方法测定IgG类抗双链DNA (dsDNA )、抗组蛋白抗体 ,用免疫荧光法检测抗核抗体核型和免疫复合物沉积 ,用免疫印迹法测定抗核抗体谱 ,在光镜下检测肾损伤及电镜下检测肾小球沉积物 ,用考马斯亮蓝法检测尿蛋白含量。结果显示 ,活性染色质能诱导IgG类抗dsDNA、抗组蛋白等多种抗核抗体生成 ,且肾小球有显著免疫复合物沉积和蛋白尿形成。该实验表明 ,活化淋巴细胞的染色质是诱导SLE发生的真正自身免疫原。

Induction of Anti-nuclear Antibodies and Renal Demages by Immunization with Active Chromatin

The purpose of this study was to make a attempt to find the real immunogen inducing the production of antinuclear antibodies and the formation of renal demages BALB/c mice were immunized with active chromatin isolated from con A activated syngeneic spleen cells The patterns of anti nuclear antibodies and the immune complexes in glomeruli were determined by immunofluorescent staining,and the peptides of extractable nuclear antigen(ENA) were determined by immunoblot assay The resultant deposits in glomerli and the renal demages were observed under light and electron microscopy,while the concentration of urinary proteins were detected by Coomassie brilliant blue assay It was found that mice immunized with active chromatin appeared to have glomerolonephritis,showing deposits of IgG immune complexes in glomeruli and in the matrix of the mesangial region and the formation of urinary proteins These results suggest that chromatin is the real immunogen of inducing the formation of anti dsDNA and other autoantibodies,and these autoantibodies might result in the renal demages