芹菜甲素对拟痴呆大鼠脑神经元保护机制的研究

目的：探讨芹菜甲素对拟老年性痴呆（AD）大鼠脑神经元的保护机制。方法：给予D-半乳糖注射和三氯化铝灌胃建立慢性中毒型痴呆大鼠模型；在制备AD模型同时用芹菜甲素灌胃；用Y型迷宫试验观察其行为学改变；用免疫组化技术检测大鼠静脉血液中超氧化歧化酶（T—SOD）及丙二醛（MDA）活性的变化；用荧光标记法测定脑细胞内游离钙离子的浓度；采用流式细胞技术、激光共聚焦技术检测神经元细胞凋亡；并分别与对照组比较，观察芹菜甲素对大鼠神经元病变、学习记忆能力的影响。结果：模型组大鼠海马和皮质具有典型的细胞凋亡特征，T—SOD活性降低，MDA含量升高；而芹菜甲素治疗组海马和皮质神经细胞凋亡数减少，T—SOD活性升高，MDA含量降低（P〈0．05或P〈0．01）。结论：芹菜甲素具有明显抗氧化作用和抑制神经细胞凋亡作用，其改善AD大鼠学习记忆功能可能是通过多个环节整体治疗来实现的。

A study on the mechanism of Donepezil's protection in neuron of hippocampus in mice with Alzheimer's disease

Objective： To study the mechanism of Donepezil＇s protection in neuron of hippocampus in mice with AD. Methods： The mice were given to D-galactolipin and chlorinate aluminium by intragastric administration to establish chronic Alzheimer＇s disease rat models, then Donepezil was given by intragastric administration. The behavioral abnormalities were investigated by Y type maze test. The expressions of T-SOD and MDA were measured by a immunohistochemistry technique. The performance and learning ability were observed. Results：Compared with model group, the performance records of learning and memory in Donepezil group were better （all P〈0.01）, the activity of T-SOD were decreased clearly （all P〈0.05）, and the expression of MDA was increased evidently （P〈0.01）. Conclusion： Donepezil has functions of neuroprotective effect, improve learning and memory ability of mice with AD,it may be involved in the mechanisms of multi approaches.