钌红对严重烧伤早期心肌能量代谢的影响

目的 探讨钌红对严重烧伤早期心肌能量代谢的影响。方法 Wistar大鼠24只，随机分为正常对照组、烧伤组和烧伤钌红治疗组。烧伤组、烧伤钌红治疗组大鼠造成30％总体表面Ⅲ度烧伤，伤后30min经腹腔补液，烧伤治疗组同时于颈外静脉推注钌红(2mg/kg体重），3h后再推注1次。伤组和烧伤治疗组动物于伤后6h活杀。测定心肌线粒体呼吸功能、Ca^2 浓度（Ca^2 ]m)及心肌组织ATP、ADP、AMP和乳酸含量。结果 钌红治疗组Ca^2 ]m较烧伤组显著降低，线粒体呼吸控制率（RCR）、Ⅲ态呼吸速率（ST3）明显升高，Ⅳ态呼吸速率（ST4）降低；钌红治疗组ATP含量较烧伤组升高100.4%，同时ADP、AMP含量明显低于烧伤组，且钌红治疗组乳酸仿较烧伤组降低53.5%。结论 钌红治疗可改善严重烧伤早期线粒体功能和心肌能量代谢。

Effects of ruthenium red on myocardial energy metabolism during t he early stage after severe burns

Objective　To investigate the effects of r uthenium red on myocardial energy metabolism during the early stage after severe burns. Methods　Wistar rats were randomized into normal control group, burns group and ruthenium red treated group (RR) (n=8 in each group). The rats in the burns group and RR group were scalded to 30% total body surface area degree Ⅲ, and 30 min later, resuscitated with Ringer's solution. Meanwh ile, ruthenium red was injected to the rats of RR in a dose of 2 mg/kg, and once again 3 h later. After rats were sacrificed at the 6th h postscalding, contents of myocard ial ATP, ADP, AMP and lactic acid, mitochondrial respiratory function and Ca 2+ concentration（［Ca2+］m） were determined. Results　Myocardial ATP content in the RR group was increa sed by 100.4% and lactic acid decreased by 53.5% as compared to the burns group. Meanwhile, mitochondrial respiratory control rate (RCR) and ST3 were higher t han those of burns group. However, ［Ca2+］m, ST4, ADP and AMP in the RR group were significantly lower than those in the burns group. Conc lusion　Ruthenium red improved myocardial energy metabolic conditions and mitochondrial function during the early stage after severe burns.