Aplicaciones de la proteómica en el laboratorio de Microbiología Clínica |
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Authors: | Juan Luis Muñ oz Bellido,Silvia Vega Castañ o,Laura Ferreira,Fernando Sá nchez Juanes,José Manuel Gonzá lez Buitrago |
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Affiliation: | 1. Departamento de Medicina Preventiva, Salud Pública y Microbiología Médica, Universidad de Salamanca, Salamanca, España;2. Servicio de Microbiología, Hospital Universitario de Salamanca, Salamanca, España;3. G.I.R. MICRAPE, Universidad de Salamanca, Salamanca, España;4. Unidad de Investigación, Hospital Universitario de Salamanca, Salamanca, España;5. Departamento de Bioquímica y Biología Molecular, Universidad de Salamanca, Salamanca, España |
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Abstract: | Matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) is rapidly becoming a new routine resource in Clinical Microbiology laboratories. Its usefulness for bacterial identification is now generally accepted, although there is still some reluctance as regards specific bacterial groups and some other microorganisms, such as moulds. There are other potential applications of this technology in Clinical Microbiology, which are beginning to be developed. A review is presented on the current data on the identification of microorganisms, including the most problematic groups, such as mycobacteria, anaerobic bacteria and moulds. We also analyse its applications for direct sample identification, its impact on pathogenic characteristics of microorganisms, and its potential epidemiological applications. Finally, we review the studies published on its applications for determining antimicrobial susceptibility, and its applications on amplicons, instead of microorganism protein extracts. |
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Keywords: | Espectrometrí a de masas MALDI-TOF Identificació n Microbiologí a |
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