The preparation of 125I-labelled bile acid ligands for use in the radioimmunoassay of bile acids. |
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| Authors: | G J Beckett J E Corrie I W Percy-Robb |
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| Affiliation: | 1. Department of Clinical Chemistry, University of Edinburgh, Royal Infirmary, Edinburgh EH3 9YW, U.K.;2. MRC Radioimmunoassay Team, 2 Forrest Road, Edinburgh EH1 2QW, ScotlandU.K. |
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| Abstract: | A general method for the preparation of 125I-labelled bile acid-histamine or 125I-labelled bile acid-tyramine conjugates is presented. The method is simple, quick and produces ligands in good yield (30%). The characteristics of a radioimmunoassay for conjugated chenodeoxycholic acid, based on an 125I-labelled ligand prepared by the method, are also described. The assay produced values for fasting serum concentrations of conjugated chenodeoxycholic acid that agree well with previous data. |
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| Keywords: | To whom correspondence should be addressed. |
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